Fish meal is the main protein source in the feed of Chinese soft-shelled turtle (Pelodiscus sinensi). Squid liver meal and soluble fish protein concentrates have high protein content and many attractive materials, and may be the alternatives to fishmeal in turtle feed. Changes in feed composition can affect the antioxidant capacity of animal. Effects of supplementation of squid liver meal and soluble fish protein concentrates in feed on the antioxidant capacity of P. sinensis, were studied in this experiment, with an attempt to explore which would be more suitable to replace fishmeal in turtle feed. Three experimental groups were designed: control group (white fish meal group, WFM), squid liver meal (SLM) group and soluble fish protein concentrates (SFP) group. Nutritional compositions of three diets were similar. For the diets of SLM and SFP, 5% white fishmeal of control diet was replaced with squid liver meal and soluble fish protein concentrates respectively. The culture experiment lasted 8 weeks. Activities of superoxide dismutase (SOD), malondialdehyde (MDA) content and total antioxidant capacity (T-AOC) of liver and serum of turtle and activities of serum alkaline phosphatase and lysozyme were determined. The experimental data were analyzed with one-way ANOVA and Duncan’s test. MDAs of liver and serum of SLM were both significantly lower than those of control group, and T-AOC of liver and serum of SLM were both higher than those of control group. No significant differences were showed in MDA of liver and serum and T-AOC of serum between SFP and control group, but liver T-AOC of SFP was significantly lower than that of control group. The results show that 5% supplementation of squid liver meal in diet can improve the turtle’s antioxidant capacity, and supplementation of squid liver meal in diet is better than soluble fish protein concentrates for Chinese turtle.