| 蒋钦杨,沈夏霜,黄钧,钟瑞鹏,郭亚芬,黄艳华,唐一波,严雪瑜.2014.齿缘摄龟线粒体基因组全序列与系统进化.动物学杂志,49(4):536-545. |
| 齿缘摄龟线粒体基因组全序列与系统进化 |
| The Complete Mitochondrial Genome of Cyclemys dentata and Phylogenetic Relationships among 20 Species of Turtles |
| 投稿时间：2013-08-14 修订日期：2014-07-09 |
| DOI：10.13859/j.cjz.201404010 |
| 中文关键词: 齿缘摄龟 线粒体全基因组 系统进化关系 |
| 英文关键词:Cyclemys dentate Mitochondrial genome Phylogenetic relationships |
| 基金项目:广西水产畜牧兽医局专项项目(桂渔牧财[2011]52号, 桂渔牧财[2012]30号) |
|
| 摘要点击次数: 3160 |
| 全文下载次数: 2409 |
| 中文摘要: |
| 采用常规PCR扩增并测序获得了齿缘摄龟线粒体DNA（mtDNA）全序列，并研究了其基因组结构特点；根据20种龟的线粒体基因组重链蛋白编码基因序列，分别利用最大简约法（MP）、最大似然法（ML）和贝叶斯法（Bayesian）构建系统进化树，探讨这些龟鳖物种之间的系统进化关系。结果显示：齿缘摄龟线粒体基因组全序列长为16489bp，GenBank登录号为JX455823；A T含量为61.51%，编码37个基因，包括13个蛋白质编码基因，2个rRNA，22个tRNA和1个控制区（D-loop），基因组成与其它龟鳖类动物相似；非编码区D-Loop长973bp，包含1个中央保守区(CD)，2个扩展终止结合序列区（ETASs），3个保守盒（CSBs）；构建的MP树、ML树和Bayesian树的拓扑结构相似，闭壳龟属7种龟聚为一枝，拟水龟属6种龟聚为一枝，齿缘摄龟与黑桥摄龟聚在一枝，3种进化树均支持这些龟鳖物种现有的分类学地位。 |
| 英文摘要: |
| In this study, the mitochondrial (mt) genome of Cyclemys dentata was firstly obtained; the relationship of 20 turtles was analyzed based on mitochondrial DNA (mtDNA). According to the mtDNA sequence of Cyclemys atripons which had been published in GenBank (No. EF067858), sixteen PCR primers were designed to amplify the mtDNA sequence of the C. dentata (Table 1). These PCR productions were inserted into the pMD-18T cloning vectors respectively, the restructured vectors were sequenced, and these sequencing fragments were spliced by Lasergene 7.0 software. The structure and gene order of C. dentata mtDNA were determined by referring to C. atripons mtDNA, and using tRNAscan-SE 1.21 to predicted tRNAs. Based on 20 turtles’ mtDNA heavy chain protein-coding genes sequences, three phylogenetic trees were constructed by Maximum parsimony (MP), Maximum-likelihood (ML) and Bayesian methods using three programs, respectively. The results indicated that the total length of mtDNA of C. dentata was 16 489 bp (GenBank No. JX455823), similar to other turtles’ mitochondrial genome, it encoded 37 genes, consisting of 13 protein-coding genes, 2 ribosomal RNA genes (12Sr RNA and 16Sr RNA), 22 transfer RNA genes (tRNAs) and a control region (D-loop) (Fig. 1, Table 2). The bases composition of C. dentata mt genome as same as that of C. atripons, its A T content was 61.51%, and A T content of 13 protein-coding genes were from 57.89% to 67.86%, their GC contents were low (Table 3). The average length of 20 turtles’ mt genomes was 16 692.5 bp, including 34.1% A, 27.0% T, 26.0% C and 12.9% G (Table 4). OL sequence was 28 bp in length; it could fold into a hairpin secondary structure which contained a 10 bp arm and an 8-base ring (Fig. 2). The D-Loop of C. dentata mtDNA contained a central domain (CD), two extended termination associated sequences (ETASs) and three conserved sequence blocks (CSBs) (Fig.3). From the Fig. 4, we knew that the MP, ML and Bayesian trees had analogical topologies, seven species of genus Cuora clustered into a branch, six species of genus Mauremys clustered into another branch, the C. dentata clustered together with the C. atripons. The analysis results of three phylogenetic trees supported the existing classification of these turtles. |
| 附件 |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
