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陈道海,霍颖娴.2015.13种双壳类贝壳的扫描电镜观察.动物学杂志,50(1):122-130.
13种双壳类贝壳的扫描电镜观察
The study on shells microstructures of thirteen species belong to bivalve
投稿时间:2013-08-28  修订日期:2014-12-31
DOI:DOI: 10.13859/j.cjz.201501015
中文关键词:  双壳类  贝壳  微观结构  扫描电镜
英文关键词:Bivalve  Shell  Microstructure  SEM
基金项目:广东自然科学基金资助项目(10152404801000013)
作者单位E-mail
陈道海 岭南师范学院环北部湾海洋药用动物保护与利用研究所 dhchen11@21cn.com 
霍颖娴 岭南师范学院生命科学与技术学院  
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中文摘要:
      采用扫描电子显微镜(SEM)对湛江地区的丽文蛤Meretrix lusoria,琴文蛤Meretrix lyrata,文蛤Meretrix meretrix,波纹巴非蛤Paphia undulata,菲律宾蛤仔Ruditapes philippinarum,杂色蛤仔Ruditapes variegata,伊萨伯雪蛤Clausinella isobellina,格粗饰蚶Anadara clathrata,泥蚶Tegillarca granosa,栉孔扇贝Chlamys farreri,尖紫蛤Sanguinolaria aauta,锈色朽叶蛤Coecella turgida,栉江珧Atrina pectinata等6科13种双壳类贝壳形貌微观结构特征进行研究。结果表明,不同贝壳的表面和横切面微观结构有一定差异。这些差异主要表现在晶体的组成和排列方式两个方面。贝壳角质层的表面形态特征有5种类型:光滑平整、颗粒状、不规则多边形、蜂窝状和沟壑状。贝壳棱柱层的晶体形状有棱柱状、短柱状、片状和不规则形状。不同贝壳的晶体有两种排列方向,垂直于横切面和平行于横切面。13种贝壳珍珠层的晶体有颗粒状、砖块状、圆形、块状和不规则的多边形。不同贝壳角质层、棱柱层和珍珠层的厚度也不同。研究贝壳微观结构之间的差异,可以为分类提供基本资料。
英文摘要:
      Research on the shells microstructures of thirteen species of bivalve which belong to six family using the scanning electron microscope (SEM). The 13 species are respectively Meretrix lusoria,Meretrix lyrata,Meretrix meretrix,Paphia undulata,Ruditapes philippinarum,Ruditapes variegata,Clausinella isobellina,Anadara clathrata,Tegillarca granosa,Chlamys farreri,Sanguinolaria aauta,Coecella turgida,Atrina pectinata. Methods: Three samples from each structure were fixed with 2.5% pentanediol, and then, cut the shells separately into 0.5 cm x 0.5 cm of small pieces. All sections of small pieces should be grinded before observed by the scanning electron microscope. Samples would be measured and taken pictures after them were observed. Resluts:The results show that the shell surface microstructures have some differences in different species. These differences mostly manifested in the compose and arrangement of the crystal. There are five types in the shell surface morphology of the corneum:smooth,granular,irregular polygon and special honeycomb structure. The crystal of the shell prismatic layer are prism-like shapes,short column,flakes and irregular shape. There are two kinds of directions in the arrangement of the crystals in different shells: perpendicular to the cross-section,another is parallel to the cross-section.The shape of the crystal in nacre of thirteen kinds of shells are granular,brick-shaped,rounded shape,massive and irregular polygons.The thickness of the corneum,prismatic layer and nacre in different shells are also different.Conclusion:Research on the microstructure of the shells,so that to provide a basis for classification.
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