Eleven bats (5 males and6 females) Myotis nipalensis were collected in Yeren Cave (31°55′8.3′′N,110°43′56.5′′E, 282maltitude), Fang County, Hubei Province, and five (1 males and4 females) M. nipalensis were collected in Linggu Cave (31°13′20.9′′N, 119°44′23.3′′E, 123maltitude), Yixing County, Jingsu Province, both on April, 2013. These were both the first records for Myotis nipalensis from Hubei and Jiangsu provinces. The feature of morphological structure and skull of the specimens was given in this paper, and comparison with specimens from Nepal and South Asia. At the same time, we used an Avisoft-UltraSoundGate 116(e) bat detector to record the echolocation calls when the bat was flying in spacious indoor conditions, and the acoustic parameters of these bats were analyzed. We also discussed the taxonomic status and distribution of this bat species in China. The specimens were kept in Guangdong Entomological Institute.