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曹力凡,李重阳,俞诗源,王敏,刘小媛.2014.X射线对小鼠胰腺组织结构及血清胰岛素含量和胰淀粉酶活性的影响.动物学杂志,49(6):804-810.
X射线对小鼠胰腺组织结构及血清胰岛素含量和胰淀粉酶活性的影响
Effects of X-ray on pancreas organization structure and the content of serum insulin and serum pancreatic amylase activity of mice
投稿时间:2013-12-06  修订日期:2014-07-14
DOI:DOI: 10.13859/j.cjz.201406003
中文关键词:  X射线  小鼠  胰腺  胰岛素  胰淀粉酶
英文关键词:X-ray  mice  pancreas  insulin  pancreatic amylase
基金项目:
作者单位E-mail
曹力凡 西北师范大学生命科学学院 lifancao@aliyun.com 
李重阳 甘肃政法学院 兰州730070  
俞诗源 西北师范大学生命科学学院 syyu006@nwnu.edu.cn 
王敏 西北师范大学生命科学学院  
刘小媛 西北师范大学生命科学学院  
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中文摘要:
      探讨X射线对小鼠胰腺组织和血清胰岛素含量及胰淀粉酶活性的影响。将成年小鼠随机分为1Gy ,3Gy,5Gy实验组和0Gy对照组(每组20只),各组小鼠给予对应剂量X射线辐射(连续3d,每d一次),用生物显微技术观察胰腺组织结构的变化,用双抗体夹心法和比色法测定胰岛素含量及胰淀粉酶酶活性的变化。结果显示X射线辐射引起小鼠胰腺组织肿胀,细胞核变大,细胞变性、坏死;1Gy辐射组小鼠胰岛素含量与对照组相比有不同程度的升高,差异显著或极显著,3Gy和5 Gy辐射组小鼠胰岛素含量与对照组相比均下降,差异显著或极显著;3Gy辐射组小鼠淀粉酶活性相比对照组均有不同程度的升高,差异显著或极显著;5Gy辐射组小鼠淀粉酶活性较对照组降低,差异极显著。结果表明X射线影响小鼠胰腺组织结构及血清胰岛素含量和血清胰淀粉酶活性。
英文摘要:
      To explore the effects of X-ray on Pancreatic tissue structure,content of serum insulin and serum pancreatic amylase activity of mice.Adult mice were randomly allocated to 1 Gy,3 Gy,5 Gy group and 0 Gy control group (20 mice in each group),each group of mice were given corresponding dose of X-ray radiation. The changes of the pancreas tissue structure were observed by optical microscope,the changes of serum insulin content and pancreatic amylase enzyme activity were determined with double antibody clip art and colorimetric method. Results showed that the X-ray radiation causes pancreatic tissue swelling,the nucleus get bigger,cell degeneration necrosis in mice;1 Gy radiation insulin levels were increased to different degree compared with the control group mice, significant or extremely significant difference;3 Gy and 5 Gy radiation mice insulin levels decreased compared with control group, significant or extremely significant difference;3 Gy radiation group amylase activity were increased to different degree than the control group in mice, significant or extremely significant difference;5 Gy radiation group amylase activity in mice decreased compared with the control group,the extremely significant difference.Results showed that the X-ray effect in pancreas organization structure and the content of serum insulin and serum pancreatic amylase activity of mice.
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