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杨莉,黄钰,王凤丽,刘静,齐亚银,张莉.2015.寒冷应激对阿勒泰羊细胞免疫及热休克蛋白70 mRNA表达的影响.动物学杂志,50(2):300-305.
寒冷应激对阿勒泰羊细胞免疫及热休克蛋白70 mRNA表达的影响
Effects of cold stress on immune system and hsp70 mRNA expression in Altay sheep
投稿时间:2014-01-16  修订日期:2014-12-29
DOI:DOI: 10.13859/j.cjz.201502019
中文关键词:  寒冷应激  热休克蛋白  白细胞介素-2  白细胞介素-4  阿勒泰羊
英文关键词:Cold stress  Hsp70  IL-2  Il-4  Altay sheep
基金项目:
作者单位E-mail
杨莉 石河子大学动物科技学院 563289492@qq.com 
黄钰 石河子大学动物科技学院  
王凤丽 石河子大学动物科技学院  
刘静 石河子大学动物科技学院  
齐亚银 石河子大学动物科技学院  
张莉 石河子大学动物科技学院 zhangli@shzu.edu.cn 
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中文摘要:
      为研究不同的寒冷应激温度对阿勒泰羊(Ovis aries)细胞免疫及热休克蛋白70(hsp70)的影响。试验采集阿勒泰羊寒冷应激前后肝脏、肺脏、脾脏、淋巴结组织及血清,采用实时荧光定量PCR中最大二阶导数法(2-ΔΔCt)对各组织中hsp70的表达量进行统计学分析,同时采用酶联免疫吸附法(ELISA)方法测定血清中白细胞介素-4(IL-4)及白细胞介素-2(IL-2)寒冷应激前后浓度变化。结果显示,在寒冷条件下阿勒泰羊各种组织中热休克蛋白hsp70的表达都有所增加,尤其是脾组织的表达增加幅度较大。ELISA方法测定阿勒泰羊在寒冷刺激后IL-4浓度发生显著下降(P=0.016),而 IL-2在冷应激后变化不显著(P=0.502),出现轻微的下调。研究表明,寒冷应激条件下,较高水平的hsp70能够保护机体免受应激的损伤。而在冷应激过程中,机体的免疫系统受到抑制。
英文摘要:
      Cold stress for the study of different temperature on the Altay Sheep (Ovis aries) cell-mediated immunity and heat shock protein 70( hsp70). Collected before and after the test cold stress Altay Sheep liver, lung, spleen, lymph node tissue and serum, using real-time quantitative PCR maximum second derivative method (2-ΔΔCt) for each tissue expression of hsp70 were statistically analyzed, while using Enzyme linked immunosorbent assay (ELISA) method for determining cold stress before and after the change of interleukin -4 (IL-4) and interleukin -2 (IL-2) concentration in the serum. The results showed that the expression of various tissues Altay Sheep heat shock protein hsp70 have increased in cold conditions, especially large increase in the expression of the magnitude of spleen tissue. ELISA method for determining the concentration of IL-4 Altay Sheep occurs after cold stimulation decreased significantly (P = 0.016), and IL-2 after cold stress did not change significantly (P = 0.502), a slight reduction. Studies have shown that, under conditions of cold stress, high levels of hsp70 stress to protect the body from injury. And in the process of cold stress, the body's immune system is suppressed.
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