| 刘建博,夏利平,徐瑞,陈思涵,应雪萍.2014.镉离子对文蛤肝胰腺超微结构的影响.动物学杂志,49(5):727-735. |
| 镉离子对文蛤肝胰腺超微结构的影响 |
| Effect of Cadmium on Ultrastructure of Hepatopancreas of Meretrix meretrix |
| 投稿时间：2014-01-25 修订日期：2014-08-25 |
| DOI：10.13859/j.cjz.201405012 |
| 中文关键词: 文蛤 镉 肝胰腺 超微结构 |
| 英文关键词:Meretrix meretrix Cadmium Hepatopancreas Cells Ultrastructrue |
| 基金项目:浙江省自然科学基金资助项目(No. LY13C04003),温州市科技创新团队项目(No. C20120007-07),国家大学生创新训练计划项目(No.201210351012),浙江省大学生科技创新项目(No.2013R424027),温州大学大学生创新创业训练计划项目(DC2013049)* 通讯作者,E-mail: xpying2008@ wzu.edu.cn；第一作者介绍 刘建博,男,本科生；研究方向：水生生物毒理；E-mail: ljbasdfgh@126.com。 |
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| 中文摘要: |
| 本文以文蛤(Meretrix meretrix)为实验对象,将其在不同浓度镉溶液(0、1.5 mg/L、3 mg/L、6 mg/L)中染毒5 d,并利用透射电镜技术分析文蛤肝胰腺细胞的超微结构变化。结果表明：镉对文蛤肝胰腺细胞超微结构的影响较大,主要表现在对内膜系统和线粒体的破坏上,其中线粒体嵴减少,肿胀变形,逐渐空泡化；粗面内质网由原先的板层状结构解体,形成许多大小不同的小泡。微绒毛脱落并逐渐减少,部分空泡化,高尔基体膜也有扩张的现象。膜性结构形成的空泡中出现沉积物,核膜出现内陷。镉离子破坏了文蛤肝胰腺细胞膜和主要细胞器的结构,影响了肝胰腺细胞的正常生理机能,随着镉溶液浓度的升高,对肝胰腺细胞结构的破坏性也增强。 |
| 英文摘要: |
| After 5 days experimental exposure of Meretrix meretrix to different dosage of cadmium solution(0、1.5mg/L、3mg/L、6mg/L), the ultrastructural changes of hepatopancreas cells of M. meretrix was observed using transmission electron microscopy. The results showed that acute cadmium poisoning made a remarkable changes of the ultrastructure of hepatopancreas cells of M. meretrix, mainly in the breakage of endomembrane system and mitochondrial. The mitochondrial became swelling and vacuolating gradually, some mitochondrial crisae disintegrated. Rough endoplasmic reticulum ( RER ) disintegrated from the original plate layered, and formed in many different sizes of vesicles. Microvilli broke off and decreased gradually, and the vacuole appeared. Cytoplasm swelled and formed vacuoles which was filled with some sediment. The nuclear membrane invaginated and the membrane of golgi apparatus expanded too. The structural of the most organelles and endomembrane system were destroyed by Cd2 solution, that affected the normal physiological function of hepatopancreas cells. With the increased concentration of cadmium solution, the cells of hepatopancreas also enhance the destruction. |
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