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蒋左玉,熊铧龙,姚俊杰.2014.葡萄糖和维生素C对普安银鲫卵黄囊仔鱼ACC、FAS及CPTⅠ活性的影响.动物学杂志,49(6):904-912.
葡萄糖和维生素C对普安银鲫卵黄囊仔鱼ACC、FAS及CPTⅠ活性的影响
Effects of Glucose and Vitamin C on Activities of ACC, FAS and CPT Ⅰ during Yolk-sac Larva Development of Carassius auratus gibelio
投稿时间:2014-03-10  修订日期:2014-08-25
DOI:DOI: 10.13859/j.cjz.201406014
中文关键词:  葡萄糖  维生素C  普安银鲫  卵黄囊仔鱼  脂肪酸合成酶  乙酰辅酶A羧化酶  肉毒碱棕榈酰转移酶I
英文关键词:Glucose  Vitamin C  Carassius auratus Linnaeus  yolk-sac larva  Fatty acid synthase  Acetyl-CoA carboxylase  Arnitine palmitoyltransferases I
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目);贵州省农业科技攻关项目(黔科合NY2008-3070)
作者单位E-mail
蒋左玉 贵州大学 动物科学学院水产科学系 gzuzyujiang@163.com 
熊铧龙 贵州大学 动物科学学院水产科学系  
姚俊杰 贵州大学 动物科学学院水产科学系 junjieyao@163.com 
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中文摘要:
      本试验旨在探究普安银鲫(Carassius auratus )卵黄囊仔鱼发育过程中ACC、FAS及CPT I活性变化及葡萄糖和维生素C溶液分别浸泡对它们的影响。采用酶学方法研究了普安银鲫卵黄囊仔鱼过程中ACC、FAS及CPT I活性变化的变化特点。结果显示:在卵黄囊仔鱼发育过程中,对照组与维生素C组中ACC和FAS活性呈上升趋势,CPT I活性呈“下降-上升”变化趋势,而葡萄糖组ACC、FAS及CPT I活性均呈上升趋势,且3种酶的活性均显著高于对照组(P<0.05)。维生素C组ACC活性在内源营养期显著高于对照组,FAS活性在混合营养期和外源营养期显著高于对照组,CPT I活性在内源营养期和外源营养期显著高于对照组(P<0.05)。研究表明:ACC、FAS及CPT I在维持普安银鲫卵黄囊仔鱼发育中脂质代谢的动态平衡起着重要作用,15g/L的葡萄糖溶液可通过调节仔鱼体内脂质代谢酶的活性而形成新的脂质代谢水平,以满足仔鱼生长发育需要;而30mg/L的维生素C对维持仔鱼发育中体内正常的脂质代谢具有重要作用。
英文摘要:
      The experiment was conducted to study the activities of fatty acid synthase,acetyl-CoA carboxylase and arnitine palmitoyltransferases and effects of glucose and vitamin c on them during yolk-sac larva development of Carassius auratus.The characteristics of fatty acid synthase,acetyl-CoA carboxylase and arnitine palmitoyltransferases activities during yolk-sac larva development of C. auratus were analyzed by the method of enzymology. The results showed that:In the group of control and vitamin c,the acitvity of atty acid synthase and acetyl-CoA carboxylase were increasing, and arnitine palmitoyltransferases activities were showed wave-like carves down-up during yolk-sac larva development of C. auratus.while the acitvity of three kinds of enzymes were increasing in the group of glucose,and they significantly higher than that of control group during yolk-sac larva development(P<0.05). In the vitamin c group, the activity of acetyl-CoA carboxylase was significantly higher than that of control group in endogenous nutrient stage, the activity of fatty acid synthase was significantly higher than that of control group between mixed nutrient stage and exogenous nutrient stage, the activity of arnitine palmitoyltransferases was significantly higher than that of control group in endogenous nutrient stage and exogenous nutrient stage,respectively.The research showed that: Fatty acid synthase,acetyl-CoA carboxylase and arnitine palmitoyltransferases play an important role in maintaining the dynamic balance of lipid metabolism during yolk-sac larva development of Carassius auratus.Glucose solution(15g/L) could regulate lipid metabolism enzyme activity form new levels of lipid metabolism to meet the needs of growth and development, and vitamin c solution( 30mg/L) has play an important role in maintaining the normal lipid metabolism. during the development of larva.
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