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安伟,肖雨,高晓华,张明辉,张崇文,邵玲,何正侃.2014.鳜源致病性荧光假单菌的分离与鉴定.动物学杂志,49(5):760-765.
鳜源致病性荧光假单菌的分离与鉴定
Isolation and Identification of Pathogenic Pseudomonas fluorescens from Siniperca chuatsi
投稿时间:2014-03-11  修订日期:2014-08-27
DOI:10.13859/j.cjz.201405016
中文关键词:    荧光假单胞菌  细菌性疾病  致病性
英文关键词:Siniperca  chuatsi, Pseudomonas  fluorescens, bacterial  identification, pathogenicity
基金项目:上海市农委青年成长计划项目[沪农青字(2014)第3-11号]
作者单位E-mail
安伟 上海市水产研究所 上海市水产技术推广站 envijoy@126.com 
肖雨 上海市水产研究所 上海市水产技术推广站  
高晓华 上海市水产研究所 上海市水产技术推广站  
张明辉 上海市水产研究所 上海市水产技术推广站  
张崇文 上海市水产研究所 上海市水产技术推广站 上海 200433  
邵玲 上海市水产研究所 上海市水产技术推广站 上海 200433  
何正侃 上海市水产研究所 上海市水产技术推广站 上海 200433  
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中文摘要:
      为确定导致鳜(Siniperca chuatsi)细菌性感染死亡的病原,从患病濒死的鳜肝中分离出一株优势菌B01,经人工回感实验确定其分离菌的致病性,并利用VITEK-2全自动微生物鉴定仪及16S rRNA序列分析对纯培养细菌进行鉴定。此外,对分离的菌株进行药敏实验。研究结果表明,菌株B01对鳜鱼具有致病性。经VITEK-2全自动微生物鉴定仪鉴定菌株B01为荧光假单胞菌(Pseudomonas fluorescens)(表1),并在紫外灯可以产生荧光(图1)。进一步的16S rRNA基因序列和系统发育树分析表明,该菌与荧光假单胞菌同源性达到了97%以上,并和(图2)。药物敏感性实验结果显示,该分离株对恩诺沙星、诺氟沙星、链霉素和四环素药物等6种药物高度敏感(表2)。
英文摘要:
      To determine the pathogenic bacterium infecting Siniperca chuatsi. Bacterium was isolated from the liver of diseased S. chuatsi and identified by the Vitek 2 Compact Identification System and 16S rRNA sequence analysis. Healthy S. chuatsi was used for experimental infection with bacterial suspension. A bacterial strain B01 was isolated from diseased S. chuatsi. Identification demonstrated that the bacterium was Pseudomonas fluorescens(Table1,Picture 2), and it’s growth characteristics was aslo studied. And the results indicated the B01 could fluoresce under ultraviolet(Picture 1). Infection with the bacterial suspension to healthy S. chuatsi could reproduce the diseased symptoms as occurred naturally and the same bacterium could be recovered from these infected Siniperca chuatsi. The susceptibility test to antibiotics demonstrated that the bacterial strain B01 was susceptible to enrofloxacin, norfloxacin, streptomycin and tetracycline(Table2). P. fluorescens is a pathogen for S. chuatsi.
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