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娄剑锋,雷世勇,竺俊全,吴雄飞.2015.岱衢洋大黄鱼染色体核型分析.动物学杂志,50(1):148-152.
岱衢洋大黄鱼染色体核型分析
Study on the Chromosome Karyotype of Pseudosciaena crocea Dai-qu Stock
投稿时间:2014-05-06  修订日期:2014-10-17
DOI:DOI: 10.13859/j.cjz.201501019
中文关键词:  大黄鱼  染色体  核型
英文关键词:Pseudosciaena crocea  chromosome  karyotype
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目)
作者单位E-mail
娄剑锋 宁波大学 765855644@qq.com 
雷世勇 宁波大学  
竺俊全 宁波大学  
吴雄飞 宁波市海洋与渔业研究院  
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中文摘要:
      为补充岱衢洋大黄鱼的细胞遗传学数据,采用植物血球凝集素(8~10μg/g)及秋水仙素(1~2μg/g)活体腹腔注射培养,头肾细胞制片、空气干燥法制作染色体标本,显微观察分析了象山港网箱养殖的岱衢洋大黄鱼的染色体核型。结果显示,岱衢洋大黄鱼二倍体染色体数目为48,核型公式为2n=24st 24t,NF=48,染色体相对长度最长为5.53,最短为2.60,未发现异型性染色体和随体。本研究的岱衢洋大黄鱼染色体核型与以往报道的大黄鱼核型存在差异。
英文摘要:
      In order to supplement P. crocea Dai-qu Stock cytogenetic data, the karyotype of net-cultured P. crocea in Xiangshan Bay was studied by microscopy. PHA (8-10μg/g) and colchicine (1-2μg/g) were injected in vivo and kidney cells were subsequently collected. Then, the cells were subjected to low permeating and Carnoy’s fixation. Finally, air-drying method was performed to make chromosome samples. The results showed that the diploid chromosome number of P. crocea Dai-qu Stock was 2n=48 and its karyotype formula was 2n=24st 24t with NF=48. The relative length of chromosomes ranged from 2.60±0.43 to 5.53±0.70 in P.crocea Dai-qu Stock. Neither sex chromosome nor satellite chromosome was found. In conclusion, The karyotype of P. Crocea in this study differs from previous report.
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