| 李磊,蒋玫,沈新强,王云龙,吴庆元,牛俊翔,许高鹏.2015.苯并[a]芘暴露对三疣梭子蟹鳃、肝胰腺组织毒理学指标的影响.动物学杂志,50(2):252-261. |
| 苯并[a]芘暴露对三疣梭子蟹鳃、肝胰腺组织毒理学指标的影响 |
| Effects of benzo[a]pyrene exposure on the toxicology parameter in gill and liver of Portunus trituberculatus |
| 投稿时间：2014-05-27 修订日期：2015-03-02 |
| DOI：DOI: 10.13859/j.cjz.201502011 |
| 中文关键词: 三疣梭子蟹 苯并[a]芘 鳃 肝脏 毒理学指标 |
| 英文关键词:benzo[a]pyrene Portunus trituberculatus gill liver toxicology parameter |
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| 中文摘要: |
| 研究了三疣梭子蟹(Portunus trituberculatus)在对苯并[a]芘(benzo[a]pyrene，BaP)的富集(15 d)、释放(15 d)过程中鳃、肝脏的四种毒理学指标(EROD、GST、SOD、MDA)的响应。结果显示：在富集阶段，第1 d时 0.05 μg/L实验组和0.45 μg/L鳃、肝脏的各毒理指标即均显著受到诱导(P<0.05)，诱导程度与暴露浓度成正相关。而后鳃、肝脏的EROD、GST活性以及鳃的SOD活性达到峰值后下降，肝脏的SOD活性以及鳃、肝脏的MDA含量则持续增加。鳃的EROD、GST、SOD活性到达峰值时间早于肝脏，其活性以及MDA含量也低于肝脏。在释放阶段，0.45 μg/L实验组鳃的SOD活性，两个实验组肝脏的SOD活性均依然显著高于对照组水平(P<0.05)，其余各浓度实验组鳃、肝脏均能恢复到对照组水平(P>0.05)。实验结果表明，三疣梭子蟹的鳃对BaP的敏感性比肝脏更强，但均具有一定的恢复能力。 |
| 英文摘要: |
| Benzo[a]pyrene (BaP) is one of the most important pollutants in the marine environment. The effects of benzo[a]pyrene on detoxifying enzyme activities (EROD, GST), antioxidant enzyme activities and (SOD) and lipid peroxidation levels (LPO) with a BaP exposure period (fifteen days) and elimination period (fifteen days) in gill and liver of Portunus trituberculatus were studied. The results showed that the activities of four toxicology parameters were significantly induced by BaP in the first day (P<0.05) and exhibited a direct correlation with the concentration of BaP. From an overall perspective, the activities of EROD, GST in the liver and the activities of SOD in gill declined after the peaks, while the activities of SOD in liver and content of MDA in liver and gill continued to rise. The time of the activities of EROD, GST and SOD reached their max value in gill was shorter than in the liver, the activities of EROD, GST, SOD and content of MDA in gill was lower than in liver, too. During the elimination period, the activities of SOD in gill with high exposure concentrations (0.45 μg/L) and in liver with both exposure concentration could not resumed to the command level (P<0.05), in contrast, the other groups could resumed to the command level (P>0.05). The results of this experiment indicated that both gill and liver of P. trituberculatus possessed resume capacity, gill was more sensitive to BaP than liver. |
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