Among the collection from the Dongjiang River, one tributary of the Pearl River in Zengcheng city and Heyuan city, Guangdong province，20 specimens of Acrossocheilus spinifer were identified as a new distribution record in the Pearl River. It could be distinguished by a combination of morphological characters: (1) 5-6 dark vertical bars on the flanks, with each bar 2 scales in width and all bars extending vertically to second scale row below lateral line, and the bars are symmetric or asymmetric; (2) the second bar positioned under the base of last simple dorsal-fin ray; (3) the last simple dorsal-fin ray strong , with small serration along posterior edge; (4) outer 2/3 part of membranes between dorsal fin rays dark. A. spinifer is diagnosed from A. beijiangensis in having 5-6 symmetric or asymmetric vertical dark bars on dorsum and flanks, each bar 1-2 scales in width (vs. in A. beijiangensis 5 symmetric vertical dark bars on flanks and each being 2-4 scales in width).The specimens were deposited in fish specimen room, School of life science, South China Normal University.