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蔡振媛,张毓,都玉蓉,苏建平,张同作.2015.基于线粒体控制区的序列变异分析青海东部甘肃鼢鼠遗传多样性.动物学杂志,50(3):337-351.
基于线粒体控制区的序列变异分析青海东部甘肃鼢鼠遗传多样性
Genetic Diversity and Population Structure of Gansu Zokors (Myospalax cansus) in Eastern Qinghai Inferred from Mitochondrial D-loop Sequences
投稿时间:2014-08-07  修订日期:2015-04-29
DOI:DOI: 10.13859/j.cjz.201503003
中文关键词:  甘肃鼢鼠  线粒体控制区  遗传多样性  种群遗传结构
英文关键词:Myospalax cansus  Mitochondria D-loop region  Genetic diversity  Population genetic structure
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目),青海省科技支撑计划项目,中国科学院仪器设备功能开发技术创新项目
作者单位E-mail
蔡振媛 中国科学院高原生物适应与进化重点实验室 中国科学院西北高原生物研究所 西宁 810001 caizhenyuan@nwipb.cas.cn 
张毓 青海省林业厅 西宁 810008  
都玉蓉 青海师范大学生命与地理科学学院 西宁 810008  
苏建平 中国科学院高原生物适应与进化重点实验室 中国科学院西北高原生物研究所 西宁 810001  
张同作 中国科学院高原生物适应与进化重点实验室 中国科学院西北高原生物研究所 西宁 810001 zhangtz@nwipb.cas.cn 
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中文摘要:
      甘肃鼢鼠(Myospalax cansus)是一种终年营地下独居生活的小型掘土类动物。本文通过测定mtDNA的控制区部分序列(530 bp)变异,分析青海东部地区8个甘肃鼢鼠地理种群遗传多样性与遗传结构。158个样本共发现26个变异位点,定义了39种单倍型,整体的平均单倍型多样性高(0.9532)、核苷酸多样性低(0.00636)。歧点分布和中性检验均说明青海东部甘肃鼢鼠种群在历史上存在着快速扩张的事件。基于邻接法构建的网络关系图中,单倍型呈星状分布,没有按地理位置形成对应类群。Nm值显示多数地理种群间基因流贫乏,AMOVA结果显示种群内与种群间遗传变异分别为48.82%、51.18%,遗传分化明显。Mantel分析表明甘肃鼢鼠的遗传分化与地理距离呈正相关,说明距离隔离对甘肃鼢鼠种群分化具有重要作用。甘肃鼢鼠的这种遗传多样性与种群遗传结构特点,可能是第四季气候变迁及地下挖掘生活方式下较小的迁移扩散能力共同作用的结果。
英文摘要:
      Gansu zokors (Myospalax cansus) are small, solitary, subterranean rodents that inhabit the Loess Plateau in China. The genetic diversity and population genetic structure of M. cansus were determined by analyzing the sequence variation of a 530 bp fragment of the mitochondrial D-loop region in 158 natural individuals from eight locations in eastern Qinghai. Overall, 26 polymorphic sites were identified and 39 haplotypes were defined. Genetic diversity estimates revealed extensive haplotype diversity (0.9532) and limited nucleotide diversities (0.00636) in all populations. Analysis of neutral evolution and mismatch distribution implied that M. cansus might have experienced a recent population expansion. The median-joining network was star-like throughout the studied range of M. cansus and showed that most individuals from different populations were highly interconnected with each other and did not exhibit reciprocal monophyly. Population differentiation among populations was confirmed by the percentage of variation among populations and within populations, which were 51.18% and 48.82%, respectively. Nm analyses indicated that the gene flow was limited among sampled populations. A Mantel test revealed significant positive correlation between genetic distances, expressed by FST, and geographical distances among populations, excluding the PA1 population, suggesting that distance isolation plays a remarkable role in genetic differentiation. In the present study, the available data suggested that the Quaternary climatic oscillation and the weak dispersal ability of subterranean animals have shaped the peculiar population genetic diversity and genetic structure of Gansu zokors.
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