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李淑兰,张欣,张婷婷.2015.黑龙江林蛙胃肠道5-羟色胺细胞的胚后发生.动物学杂志,50(4):635-642.
黑龙江林蛙胃肠道5-羟色胺细胞的胚后发生
5-Hydroxytryptamine Cells in Gastrointestinal Tract during the Postembryonic Development of Rana amurensis
投稿时间:2014-09-05  修订日期:2015-05-15
DOI:DOI: 10.13859/j.cjz.201504017
中文关键词:  黑龙江林蛙  胃肠道  5-HT细胞  胚后发生
英文关键词:Rana amurensis  Gastrointestinal Tract  5-HT cells  postembryonic development
基金项目:黑龙江省教育厅科研项目(No.12521162);
作者单位E-mail
李淑兰* 哈尔滨师范大学生命科学与技术学院 478970204@qq.com 
张欣 哈尔滨师范大学生命科学与技术学院  
张婷婷 哈尔滨师范大学生命科学与技术学院  
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中文摘要:
      本文采用ABC免疫组织化学法,对黑龙江林蛙(Rana amurensis )胚后发育各时期胃肠道中5-羟色胺(5-Hydroxytryptamine,5-HT)细胞的发生过程进行研究,结果显示:黑龙江林蛙胃中的5-HT阳性细胞最早在第38期被检测到,小肠和大肠最早出现在第41期。随着胃肠道发育的日渐完善,5-HT细胞的分布位置发生了很大的变化,从开始的上皮细胞之间,到上皮基部或腺泡上皮之间。细胞形态变化不明显,有圆形、椭圆形、锥体形和长梭形等。推测5-HT阳性细胞的出现时间、分布位置及形态与黑龙江林蛙的生长发育需要、胃肠道各段的功能是相适应的。
英文摘要:
      A immunohistochemical (ABC methods) methods was used to study the 5-Hydroxytryptamine (5-HT) cells generant process in the Gastrointestinal Tract of the postembryonic development of Rana amurensis . The result show that the 5-HT positive cells were first found in the stomach at stage 38,and first found in the Small and large intestine at stage 41.With the development of the gastrointestinal tract, the location of the 5-HT cells were changed a lot, between the mucosal epithelium, the basal part or the between alveolar epithelial cells. But the shape of cells not have clearly changed, they were mainly round, ellipse, cone-shape and shuttle-shaped.The occurs time of 5-HT cells,the location and shape were conformable with needs of growth, and the function of each segment of the gastrointestinal tract.
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