| 龚小玲,王米雪,汪德海,鲍宝龙.2015.牛蛙外周血细胞的形态学特点.动物学杂志,50(4):591-599. |
| 牛蛙外周血细胞的形态学特点 |
| The morphological characteristics of the peripheral hematocytes in bullfrog, Rana catesbeiana |
| 投稿时间：2014-09-28 修订日期：2015-04-23 |
| DOI：DOI: 10.13859/j.cjz.201504011 |
| 中文关键词: 牛蛙 外周血细胞 形态 数量 |
| 英文关键词:Bullfrog Peripheral Hematocytes Morphology Quantity |
| 基金项目:国家自然科学基金(41176108)和上海教委创新重点项目(14ZZ145) |
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| 中文摘要: |
| 牛蛙（Rana catesbeiana）外周血细胞的形态学尚缺乏详细研究，本研究对雌雄牛蛙外周血细胞的组成、形态、大小和数量进行了观察和统计。牛蛙外周血细胞由红细胞、白细胞以及血栓细胞组成，其中红细胞体积最大，平均大小（长径 × 短径）为（25.68 ± 1.88）μm ×（16.49 ± 1.53）μm，扫描电镜下发现红细胞表面光滑；血栓细胞呈卵圆形或纺锤形，其体积最小，平均大小为（8.62 ± 1.04）μm ×（7.47 ± 1.11）μm；白细胞由淋巴细胞、单核细胞、浆细胞、嗜中性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞组成；扫描电镜下白细胞表面粗糙不平，有许多不规则的凸起。白细胞中淋巴细胞最多，其中小淋巴细胞约占32.66% ± 4.29%，大淋巴细胞约占6.03% ± 1.54%；嗜碱性粒细胞最少，只占4.78% ± 0.83%；浆细胞胞体大小不一，常呈椭圆形，平均大小为（23.51 ± 0.59）μm ×（22.86 ± 0.67）μm；此外，牛蛙外周血细胞中单核细胞、淋巴细胞和嗜碱性粒细胞的数量比例以及淋巴细胞和嗜碱性粒细胞的大小均有性别的差异（P < 0.05）。 |
| 英文摘要: |
| The bullfrog （Rana catesbeiana）, which belongs to Anura, Ranidae and Rana, is one of the important economic farmed animals in China. The morphologic of the peripheral hematocytes in the bullfrog has not yet received enough attention. In this study, we observed the composition and morphology of the peripheral blood cells from 20 individuals (each 10 for male and female) using Wright's staining under the light microscope or the scanning electron microscopy (SEM). The diameters of various hematocytes and their nuclei in shortest and longest axises were measured using routine methods, and the experimental date were analyzed by SPSS19.0.The results showed that the peripheral hematocytes of the bullfrog were composed of erythrocyte, leukocyte, and thrombocyte. The mean size of erythrocytes in length × width was 25.68 ± 1.88 μm × 16.49 ± 1.53 μm (Table 1). The surface of erythrocyte was smooth under the SEM (PlateⅡ). Typically, Thrombocyte was ovoid or spindle in shape (PlateⅠ) and it was the smallest blood cell with mean size were 8.62 ± 1.04 μm × 7.47 ± 1.11 μm (Table 1). Leukocytes consisted of lymphocyte, monocyte, plasmacyte, neutrophil, eosinophil and basophil. Under the SEM, the surfaces of various leukocytes were rough with many irregular protrusions (PlateⅡ). Lymphocytes including small and large lymphocytes occupied higher percent, small lymphocytes were found approximately to be 32.66% ± 4.29% of the total leukocyte, large lymphocytes accounted for about 6% (Table 1). The number of the basophils was the least in the leukocytes, only accounted for about 4.78% ± 0.83% (Table 1). Plasmacytes were typically elliptical in shape (PlateⅠ), and their sizes varied obviously around the mean size 23.51 ± 0.59 μm × 22.86 ± 0.67 μm (Table 1). In addition, the quantitative proportion of the lymphocyte, basophil and plasmacyte and the size of lymphocyte and basophil were influenced by gender（P < 0.05）. |
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