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谢展望,颉志刚,胡一中,郑荣泉.2015.野生棘胸蛙消化器官蛋白酶的分布及酶活力.动物学杂志,50(5):765-772.
野生棘胸蛙消化器官蛋白酶的分布及酶活力
Distribution and Activity of Protease in Digestive Organs of Wild Giant Spiny Frog, Quasipaa spinosa
投稿时间:2014-10-24  修订日期:2015-06-17
DOI:DOI: 10.13859/j.cjz.201505013
中文关键词:  棘胸蛙  消化系统  蛋白酶  酸碱度  温度
英文关键词:Rana spinosa  Digestive system  Protease  pH value  Temperature
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目)
作者单位E-mail
谢展望 浙江师范大学化学与生命科学学院生态研究所 金华 404690966@qq.com 
颉志刚 浙江师范大学化学与生命科学学院生态研究所 金华  
胡一中 浙江师范大学化学与生命科学学院生态研究所 金华 huyizhong@zjnu.cn 
郑荣泉 浙江师范大学化学与生命科学学院生态研究所 金华 321004  
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中文摘要:
      本研究在不同pH和温度条件下以离体方式对野生棘胸蛙( Quasipaa spinosa)不同消化器官的蛋白酶活力进行了分析。结果表明,食道、胃、胰的蛋白酶活力受到酶体系pH的显著影响( P < 0.05),且随pH升高呈现典型的单峰型活力曲线。食道和胃的蛋白酶活力在pH为1.5时达到峰值,胰的酶活力在pH为9.6时达到峰值,而肠道酶活力在pH为7.4时达到峰值。各消化器官蛋白酶活力具有明显的温度依赖性( P < 0.05),酶活力随温度升高也均呈出典型的单峰型活力曲线,不同消化器官的最大酶活力温度分别为,食道50℃、胃50℃、胰45℃、前肠45℃、后肠45℃、直肠45℃。在最大酶活力的pH和30℃条件下,各消化器官蛋白酶活力由高到低依次为胰、食道、胃、直肠、前肠、后肠。由此可见,蛋白酶在棘胸蛙消化系统的分布具有明确的规律性,且不同来源的蛋白酶需要在特定pH和温度下才能表现出最大的反应活性。
英文摘要:
      This study investigated protease activities in different digestive organs of wild giant spiny frog (Quasipaa spinosa) in the range of pH 0.5 to 10.0 at 30℃ and reaction temperature 15 to 60℃ at optimum pH in vitro. The protease activity was measured using the Folin-phenol method. The data were statistically analyzed by One-way ANOVA and LSD. The results showed that pH could affect protease activities of esophagus, stomach and pancreas significantly (P < 0.05), and their dynamic curves of protease activities were typically unimodal with increasing pH (Fig. 1).The protease activities of esophagus, stomach, pancreas and intestine were maximal at pH 1.5, 1.5, 9.6 and 7.4, respectively. The protease activities of digestive organs showed an obvious temperature dependence (P < 0.05), and the curves were also typically unimodal (Fig. 2). The protease activities of esophagus, stomach, pancreas, foregut, hindgut and rectum attained maximum value at temperature 50℃, 50℃, 45℃, 45℃, 45℃ and 45℃, respectively. Under the conditions of 30℃ and optimal pH mentioned above, digestive organs were arranged in order of descending protease activities: pancreas, esophagus, stomach, rectum, foregut and hindgut. In conclusion, there obviously exists a regular protease distribution in digestive system of this species, and the enzymes from different organs can show maximal activities at specific pH values and temperatures.
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