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梁娜,彭西,袁施彬,陈科杰.2015.幼龄红嘴相思鸟小脑皮层组织学结构.动物学杂志,50(5):744-751.
幼龄红嘴相思鸟小脑皮层组织学结构
The histological observation on the development ofcerebellar cortex in Leiothrixlutea after early hatching
投稿时间:2014-11-06  修订日期:2015-08-27
DOI:DOI: 10.13859/j.cjz.201505010
中文关键词:  红嘴相思鸟,小脑皮层,发育,组织学
英文关键词:Leiothrixlutea, cerebellar  cortex, development, histology
基金项目:西南野生动植物资源保护教育部重点实验室开放“热应激对笼养红嘴相思鸟组织结构的影响及壳聚糖的抗应激效应”(XNYB11-4,2011-20)。
作者单位E-mail
梁娜 四川农业大学动物医学院 liangna19911314@163.com 
彭西 四川农业大学动物医学院 pengxi197313@163.com 
袁施彬 西华师范大学生命科学学院  
陈科杰 四川农业大学动物医学院  
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中文摘要:
      为了探讨不同日龄幼龄红嘴相思鸟(Leiothrix lutea)小脑皮质的组织学结构变化,本文分别以1、5、9日龄幼龄红嘴相思鸟为研究对象,通过H.E.和甲苯胺蓝法进行染色,光镜下观察红嘴相思鸟小脑冠状切面的显微结构。结果显示,1日龄时小脑皮层由外颗粒层(EGL)、浦肯野细胞层(PCL)和内颗粒层(IGL)3层构成,外颗粒层较厚且清晰,而浦肯野细胞层和内颗粒层界限不清楚;5日龄和9日龄时小脑皮质均可见外颗粒层、分子层(ML)、浦肯野细胞层和内颗粒层4层结构。对不同日龄红嘴相思鸟小脑皮质各层厚度进行单因素方差分析显示,随日龄增长,小脑皮质、分子层和内颗粒层厚度极显著增厚(P < 0.01),浦肯野细胞体积也极显著增加(P < 0.01);外颗粒层厚度变化不明显(P < 0.01),呈现先增厚后变薄的趋势,与皮质厚度的比例逐渐减小。研究表明,幼龄红嘴相思鸟在出生后,随日龄增长,小脑皮质层逐渐发育成熟。内颗粒层与外颗粒层的相对变化规律表明内颗粒层细胞是由外颗粒层细胞迁移而来。
英文摘要:
      To study the histological changes of cerebellar cortex of immature Red-billed Leiothrix(Leiothrix lutea) at different days of age, brains were sampled from Red-billed Leiothrixs at 1, 5 and 9 days of age. Through H.E. and toluidine blue staining, the coronal section of the cerebellum was observed to analyze the changes of cellular and postnatal structural patterns by microscope, and all the data were dealed with one-way ANCOVA. The results are as follow:The H.E. staining shows that the cerebellar cortex of Red-billed Leiothrix at 1 day of age consisted of three layers, namely external granular layer (EGL), unclear Purkinje cell layer (PCL) and internal granular layer (IGL), the external granular layer was obvious, but the margin of Purkinje cell layer and internal granular layer was not distinct (Fig1a, 1d). At 5 and 9 days of age, a clear four-layer structure was observed, including external granular layer, molecular layer (ML), Purkinje cell layer and internal granular layer (IGL)(Fig1b ~ 1c, 1e ~ 1f). With the increase of the age, the thickness of the cortex, molecular layer and internal granular layer were significantly increased (P < 0.01). Toluidine blue staining shows clearly that the size of Purkinje cell was significantly increased with the increase of the age. At 1 day of age, the Purkinje cell was small with pyriform and the nissl body was dark-colored(Fig2a, 2d), at 5 days of age, the Purkinje cells were enlarged(Fig2b, 2e). At 9 days of age, the size of the Purkinje cell was increased and the boundary between Purkinje cell layer and internal granular layer was apparent (Fig2c, 2f). Although the thickness of external granular layer thins after a slightly increase and has no significant change (P > 0.05) (Table 1), there was a reduced proportion of the external granular layer to the cortex(Table 2). Also the index of internal granular layer to the cortex was increased(Table 2). The results showed that the histological structure of cerebellar cortex was gradually matured in Red-billed Leiothrixs after early hatching. The changes of external granular layer and internal granular layer suggested that the granular cells in internal granular layer might migrate from external granular layer.
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