| 杨淞,符红梅,赵柳兰,肖青,王艳,杜宗君.2015.黄石爬鮡外周血液指标特征的观察.动物学杂志,50(6):922-930. |
| 黄石爬鮡外周血液指标特征的观察 |
| Characteristics of Peripheral Haematological Parameters in Euchiloglanis kishinouyei |
| 投稿时间：2015-01-05 修订日期：2015-11-11 |
| DOI：DOI: 10.13859/j.cjz.201506011 |
| 中文关键词: 黄石爬鮡 外周血液指标 显微结构 细胞化学 |
| 英文关键词:Euchiloglanis kishinouyei Peripheral Haematological Parameters Microstructure Cytochemical Characteristics |
| 基金项目:四川省农业厅水产局高原鱼类资源保护与开发，四川农业大学“双支计划”项目(NO.03750202) |
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| 中文摘要: |
| 黄石爬鮡（Euchiloglanis kishinouyei）属于较为原始的高原鱼类。通过生化分析仪测定红细胞数及血红蛋白含量，Wright’s染色及细胞化学方法，即过碘酸雪夫（PAS）、酚氧化酶（PO）、苏丹黑B（SBB）和过氧化物（POX）染色，观察其外周血细胞的显微结构及细胞化学特征。黄石爬鮡的红细胞数为（0.55 ± 0.06）×1012 个/L，血红蛋白含量为（73.00 ± 5.57） g/L；血细胞中红细胞占98.03%，且其细胞体积较大，白细胞中血栓细胞占比例最多，为37.06%，异嗜性粒细胞最少，为9.64%，异嗜性粒细胞分为Ⅰ型、Ⅱ型和Ⅲ型。细胞化学染色显示，红细胞均呈阴性，白细胞存在染色特性差异，其中白细胞过碘酸雪夫（PAS）染色均为阳性，过氧化物（POX）染色除I型和III型异嗜性粒细胞外均为阴性。 |
| 英文摘要: |
| Euchiloglanis kishinouyei is an ancestral mountain fish, however, as a result of water project construction and over?shing, populations of the fish have declined extremely in abundance in the last decades. So,the protection of fish resource becomes particularly urgent and it is high time to take a series of protective measures. The purpose of this study was to investigate the morphological and cytochemical characteristics of peripheral blood cells of the fish by light microscopy, to provide information for domestication and disease cure and to accumulate basic data for hematology of the fish. In this work, red blood cell count (RBC) and haemoglobin concentration (Hb) were determined by fully automatic biochemical analyzer and blood smears stained with Wright’s and cytochemical stainings, including periodic-acid schiff (PAS), phenoloxidas (PO), sudan black B (SBB) and peroxidase (POX).Data are presented as mean ± standard deviation.The results showed RBC was (0.55 ± 0.06)×1012 cell/L and hemoglobin concentration was (73.00 ± 5.57) g/L, and erythrocytes and four types of leukocytes: thrombocytes, lymphocytes, monocytes and heterophils, were identified in E. kishinouyei peripheral blood (Fig. 1). Additionally, erythrocytes were the most abundant and largest cells in smears of the peripheral blood, accounting for 98.03%. Thrombocytes were the predominant leukocyte, accounting for 37.06% of all leukocytes, whereas heterophils were rare, only accounting for 9.64% (Table 1).According to the size and morphologic features of heterophils and nucleus and quantities of cytoplasmic granules, type Ⅰ,Ⅱ and Ⅲ of heterophils were recognized. Erythrocytes were negative for periodic-acid schiff (PAS), phenoloxidas (PO), sudan black B (SBB) and peroxidase (POX), and all leukocytes were positive for periodic-acid schiff (PAS) (Fig. 2). Type Ⅰ and Ⅲ of heterophils were strong positive for peroxidase (POX), while thrombocytes, lymphocytes, monocytes and type Ⅱ of heteropils were negative for peroxidase (POX) (Table 2). |
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