In order to study the effect of amphibians mortality caused by road traffic, one sample transect was choose separately on the Ring Changbai Mountain Scenic highway, Toudao road and Baishan road in the Changbai Mountain National Nature Reserve from 2007 to 2014. Amphibians road mortality species richnes, number, and location were recorded by direct counting method with the total length of sample survey was 2 441 km and 257 times. The results showed that 7 910 amphibians, belonging to 5 species were recorded as road killed with the average mortality was 27.9/ l0km. The most vulnerable species were Chinese Brown Frog (Rana chensinensi), Oriental Fire-bellied Toad (Bombina orientalis) and Asiatic toad (Bufo gargariza), for the mortality accounting for 96.3% of the total mortality. August was peak period for road mortality of amphibians, and then concentrated in breeding season from April to June. In the view of yearly mortality, all amphibian species tended to decline tendency except for Chinese Brown Frog and Asiatic toad, which only had small extent variation in different years. The monthly mortality was significant correlation with the monthly traffic volume (r=0.913,p=0.004). Accordingly, some protective measures of amphibians were provided.