| 张浩,柏青杨,杜凤霞,李玉霞,杨秀静.2016.不同发育期贝居腹盾吸虫体内乙酰胆碱酯酶分布特征.动物学杂志,51(4):655-662. |
| 不同发育期贝居腹盾吸虫体内乙酰胆碱酯酶分布特征 |
| Distribution of Acetylcholinesterase at Different Development Stages of Aspidogaster conchicola |
| 投稿时间：2015-03-24 修订日期：2016-07-09 |
| DOI：DOI: 10.13859/j.cjz.201604017 |
| 中文关键词: 贝居腹盾吸虫 发育 乙酰胆碱酯酶 |
| 英文关键词:Aspidogaster conchicola development acetylcholinesterase |
| 基金项目:黑龙江省教育厅科学技术研究项目(编号：12531781) |
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| 中文摘要: |
| 为了研究贝居腹盾吸虫不同发育阶段生殖器官乙酰胆碱酯酶（AChE）的分布变化，探索神经递质乙酰胆碱（ACh）在吸虫生殖活动中的作用，采集河蚌体内的贝居腹盾吸虫, 活体压片后镜下观察，根据虫体的内部结构分类为幼虫组和成虫组。采用醋酸洋红染色和乙酰胆碱酯酶组织化学染色，对比分析不同发育阶段虫体内乙酰胆碱酯酶（AChE）分布的差异。结果显示，睾丸及阴茎囊最先出现AChE阳性反应，随后是卵黄腺、输卵管和子宫末段。而吸虫的附着、消化、排泄器官在发育早期即出现阳性反应，并伴随虫体发育全过程；神经系统发育较早，而以成虫期的中枢神经结构发育最完备。结果提示，神经递质ACh可能参与该吸虫的生殖活动。 |
| 英文摘要: |
| To study the distribution change of acetylcholinesterase(AChE) of Aspidogaster conchicola reproductive organ in different development stages and explore the roles of neurotransmitter Ach in the trematode reproductive activity，Aspidogaster conchicola was collected from the Anodonta and was divided into larval stage groups and adult stage groups according to internal strucutre of the trematode under microscope after maked into living body tabletting. The acetic acid Carmine staining and the histochemical staining were used to display the distribution of AChE in two stages of the trematode and distributional difference of AChE was contrasted. The result displayed the testicles and penis sac of the trematode first appears positive reaction,followed by the vitelline gland, the fallopian tubes and the last paragraph of uterus. And its attached, digestive and excretory organs appear the AChE positive reaction in the early stage and its whole development process. The brain ganglion and ventral nerve trunk is clearly visible at each stage of the trematode and the central nerve structure is the most perfect in the maturing stage. It hint neurotransmitter Ach may participate in the trematode reproductive activity. |
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