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孟婷婷,王倩,葛宝,潘巧琳,李金钢,何建平.2017.甘肃鼢鼠哈氏腺组织结构及其抗氧化能力.动物学杂志,52(2):283-293.
甘肃鼢鼠哈氏腺组织结构及其抗氧化能力
The Harderian Gland of the Gansu Zokor (Myospalax cansus): A Structural and Antioxidant Study
投稿时间:2015-04-17  修订日期:2016-11-09
DOI:10.13859/j.cjz.201702012
中文关键词:  甘肃鼢鼠  低氧  哈氏腺  抗氧化酶  丙二醛
英文关键词:Gansu Zokor, Myospalax cansus  Hypoxia  Harderian Gland  Antioxidase  Malondialdehyde (MDA)
基金项目:陕西省自然科学基金项目(No. 2016JM3024)
作者单位E-mail
孟婷婷 西安培华学院 282009333@QQ.com 
王倩 陕西师范大学生命科学学院 西安 710062  
葛宝 陕西师范大学生命科学学院  
潘巧琳 陕西师范大学生命科学学院  
李金钢 陕西师范大学生命科学学院  
何建平 陕西师范大学生命科学学院 hejianping@snnu.edu.cn 
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中文摘要:
      为探讨甘肃鼢鼠(Myospalax cansus)哈氏腺的结构特征及其在低氧应激下的抗氧化能力,用组织解剖学方法观察甘肃鼢鼠哈氏腺整体及其显微结构,分光光度计测定哈氏腺低氧应激前、后超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)活性及丙二醛(MDA)含量。结果显示,甘肃鼢鼠哈氏腺肥大,包围在眼周,位于颧骨下的颞窝,为管泡状腺体,由柱状细胞构成,依胞质中分泌物含量分为厚细胞和薄细胞。常氧下,甘肃鼢鼠超氧化物歧化酶和过氧化氢酶活性显著高于SD大鼠(Rattus norvegicus),但谷胱甘肽还原酶活性显著低于SD大鼠;在低氧应激4 h后,甘肃鼢鼠超氧化物歧化酶和过氧化氢酶活性迅速升高,显著高于SD大鼠,谷胱甘肽还原酶的活性在低氧2、4和6 h无显著性变化,但均显著低于SD大鼠;在低氧8 h后,甘肃鼢鼠谷胱甘肽还原酶的活性较低氧2 ~ 6 h显著升高。甘肃鼢鼠丙二醛含量在常氧和低氧应激中均显著低于SD大鼠。结果说明,甘肃鼢鼠在低氧应激后,哈氏腺通过提高抗氧化酶超氧化物歧化酶和过氧化氢酶活性,清除低氧诱导产生的多余自由基,谷胱甘肽还原酶在抗氧化中不起主要作用。地下鼠甘肃鼢鼠抗氧化模式与地面鼠明显不同。
英文摘要:
      To the present study investigated the structure of Harderian gland and its antioxidases during low oxygen stress in Gansu Zokor (Myospalax cansus). The overall gross anatomic structure of Harderian gland was described and its microstructure was observed by paraffin H.E staining. The activities of antioxidases in Harderian gland including superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and content of malondialdehyde (MDA) in normoxia and hypoxia were measured by a spectrophotometer. The data were analyzed by the single factor analysis of variance using SPSS13.0. The results were as follows: Harderian gland of Gansu Zokor was large (Table 1), surrounding around the eye and lying in the temporal fossa under the jugal bridge (Fig. 1b). It was a tube vesicular gland and composed of columnar cells (Fig. 2a). According to the active state,the cells could be divided into thick and thin cells (Fig. 2c). In normoxic condition, the activities of catalase (CAT), superoxide dismutase (SOD) were significantly higher than those in Rat (Rattus norvegicus), and the activity of glutathione reductase (GR) was significantly lower than that of SD Rat. The activities of catalase (CAT), superoxide dismutase (SOD) increased rapidly and significantly, higher than those in SD Rat at low oxygen after 4 hours. The activity of glutathione reductase (GR) showed no significant change at low oxygen after 2﹣4 hours, and significantly lower than that of SD Rat. The activity of glutathione reductase (GR) was significantly higher after 2﹣6 hours (Table 1). Significantly lower malondialdehyde (MDA) content was observed in Gansu Zokor than in SD Rat (Table 2). The activities of catalase (CAT), superoxide dismutase (SOD) in Gansu Zokor increased rapidly and significantly, higher than those in SD Rat at low oxygen after 4 hours. The same result was observed in activity of glutathione reductase (GR) after 8 hours. Significantly lower malondialdehyde (MDA) content was found than in SD Rat (Table 2). The results show that Gansu Zokor can maintain normal activity through increasing the activity of antioxidase, removing excessive free radicals induced by low oxygen condition and adapt to the environment. Glutathione reductase (GR) dose not paly a major role in antioxidant function. Underground Rodents antioxidant model was obviously different from that of the ground Rats.
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