In this study, histologic methods were used to study the organizational structure of female ovary development induced by eyestalk ablation in red swamp crayfish (Procambarus clarkii). Total of 60 female crayfishes in phase II were selected and the experiment was divided into four groups as well as two time points (group 1: control for 15 days; group 2: control for 30 days; group 3: treatment for 15 days; group 4: treatment for 30 days). The data was analyzed using SPSS 19.0 statistical software. Our results show that 30 days after eyestalk ablation, the individual body-length increased significantly, from (11.50 ± 2.30) cm increased to (18.20 ± 3.40) cm(P<0.05)(Table 1); GSI index growth from 4.56% ± 2.81% to 8.05% ± 2.51% (P<0.05), as well as body-weight and chelicerae-long index also increased(P>0.05). 30 days after eyestalk ablation, the oocytes observed by electron microscope were quickly developed from phase II through stage III to VI stage(Table 2), Oocyte volume becomes larger, more rounded; Vacuole compressed smaller; yolk granules accumulated gradually accelerated, finally fill the entire intracellular space; cytoplasm in follicular cells becomes thinner(Figure 1). These results indicated that eyestalk ablation could significantly accelerate the yolk granules in oocytes of accumulation, and speed up the individual growth.