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邹远超,文正勇,岳兴建,王永明,覃川杰,陶敏,李斌,谢碧文,王淯,齐泽民.2016.泉水鱼的细胞遗传学分析.动物学杂志,51(1):66-72.
泉水鱼的细胞遗传学分析
Cytogenetic Analysis of Pseudogyrinocheilus prochilus
投稿时间:2015-05-04  修订日期:2015-12-29
DOI:DOI: 10.13859/j.cjz.201601008
中文关键词:  泉水鱼  染色体  核型  DNA含量
英文关键词:Pseudogyrinocheilus prochilus  chromosome  Karyotype  DNA content
基金项目:四川省教育厅科技创新科研团队基金项目(No. KYTD201009), 四川省教育厅项目(No. 15ZA0285);
作者单位E-mail
邹远超 内江师范学院 zou3891@163.com 
文正勇 内江师范学院  
岳兴建 内江师范学院  
王永明 内江师范学院  
覃川杰 内江师范学院  
陶敏 内江师范学院  
李斌 内江师范学院  
谢碧文 内江师范学院  
王淯 内江师范学院  
齐泽民 内江师范学院  
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中文摘要:
      以泉水鱼(Pseudogyrinocheilus prochilus)的肾为材料,采用体内注射植物血球凝集素(PHA)和秋水仙素、肾组织细胞短期培养、常规空气干燥法制备泉水鱼染色体标本,对其核型进行分析。以泉水鱼外周血细胞为样本,鸡(Gallus gallus)血细胞DNA含量为标准(2.50 pg/2c,2c指二倍体),用流式细胞仪测定了泉水鱼的DNA 含量。 结果表明:(1)泉水鱼的染色体数量为2n = 50,核型公式为12m + 14sm + 14st + 10t,总臂数NF = 76,未发现性别相关的异型染色体;(2)泉水鱼的DNA含量为鸡血对照的1.05± 0.04 倍,其绝对DNA含量为(2.62 ± 0.10)pg/2c。泉水鱼的染色体数目和DNA 含量显示出二倍体的特征。
英文摘要:
      The chromosome number and karyotype of Pseudogyrinocheilus prochilus were analyzed using a routine method including intraperitoneal injection of PHA(phyohemagglutinin)and colchicine,cultivation in vivo,and slides preparation with air—drying.While erythrocyte nuclear DNA content of P. prochilus was determined by using a flow cytometer (Beckman Coulter Inc.,USA) with serving the chicken(Gallus gallus) erythrocytes (their DNA content is 2.50 pg/2c) as reference cells. The results showed that the diploid chromosome number of P. prochilus was 2n = 50, and the karyotype of this species was 2n = 12m + 14sm + 14st + 10t,NF = 76(Fig.1 and Tab.1). The heterosomes were not observed. The diploid cellular DNA contents of P. prochilus was 2.62± 0.10 pg/2c(n=20),which was 1.05± 0.04 times to the chicken erythrocytes (Fig.2). The result of karyotype and DNA contents of P. prochilus showed that P. prochilus was diploid.
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