| 张建萍,惠晓红,杨丽丽,王强.2016.塔里木兔和家兔的消化酶活性比较研究.动物学杂志,51(1):33-38. |
| 塔里木兔和家兔的消化酶活性比较研究 |
| Comparative Study on Digestive Enzymatic Activities between Yarkand Hares(Lepus yarcandensis) and Domestic Rabbits(Oryctolagus curiculus) |
| 投稿时间：2015-06-19 修订日期：2015-12-29 |
| DOI：DOI: 10.13859/j.cjz.201601004 |
| 中文关键词: 塔里木兔(Lepus yarcandensis) 家兔(Oryctolagus curiculus) 淀粉酶 纤维素酶 脂肪酶 胰蛋白酶 |
| 英文关键词:Yarkand hare(Lepus yarcandensis) Domestic rabbits(Oryctolagus curiculus) Amylase Cellulase Lipase Trypsin |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 摘要点击次数: 1998 |
| 全文下载次数: 2067 |
| 中文摘要: |
| 为比较塔里木兔(Lepus yarcandensis)和家兔（Oryctolagus curiculus）消化能力的差异，探索塔里木兔对野外生存环境的食物的适应机制，以家兔为对照，测定了塔里木兔和家兔的胰腺及肠道的消化酶—淀粉酶（碘-淀粉比色法）、纤维素酶（3，5-二硝基水杨酸法）、脂肪酶（比浊法）及胰蛋白酶（紫外线吸收法）的活性。用 SPSS 15.0 软件对实验数据进行统计学分析。结果表明：1）塔里木兔肠道的淀粉酶活性明显高于家兔。其中，塔里木兔十二指肠、空肠及回肠的淀粉酶活性极显著高于家兔（P ＜ 0.01）。淀粉酶活性在塔里木兔和家兔空肠最高。2）塔里木兔肠道的纤维素酶活性高于家兔。其中，塔里木兔盲肠的纤维素酶活性极显著高于家兔（P ＜ 0.01），其回肠的纤维素酶活性显著高于家兔（P ＜ 0.05）。纤维素酶活性在塔里木兔和家兔盲肠最高。3）家兔胰腺和肠道的脂肪酶活性明显高于塔里木兔。其中，家兔胰腺、十二指肠、空肠及回肠的脂肪酶活性显著高于塔里木兔（P ＜ 0.05）。与消化道相比，脂肪酶活性在家兔和塔里木兔胰腺最高，肠道中空肠的脂肪酶活性最高。4）家兔胰腺和肠道的胰蛋白酶活性高于塔里木兔。其中，家兔空肠和十二指肠的胰蛋白酶活性显著高于塔里木兔（P ＜ 0.05）。胰蛋白酶活性在家兔和塔里木兔空肠最高。可见，与家兔相比，塔里木兔对淀粉类、纤维类物质有较强的消化能力，对脂肪类的消化能力较弱。其中，淀粉酶活性和纤维素酶活性升高，脂肪酶活性和胰蛋白酶活性降低。这可能是塔里木兔适应贫瘠、匮乏的食物环境的重要原因之一。 |
| 英文摘要: |
| To investigate the differences of digestive ability between yarkand hares(Lepus yarcandensis)and domestic rabbits(Oryctolagus curiculus), and hence to explore the mechanism underlying the adaptability to food environment in yarkand hares, we assayed the following digestive enzyme activities: amylase activity (Iodine starch colorimetric?method), cellulose enzyme activity (3, 5 - 2 nitro salicylic acid method), lipase activity (turbidity method) and trypsin (ultraviolet absorption method), with the samples taken from pancreas and intestinal tract of yarkand hares and domestic rabbits using domestic rabbits as control. The experimental data were statistically analyzed by SPSS 15 software.The results demonstrated that: 1) The amylase activity in intestinal tract of yarkand hares was significantly higher than those of domestic rabbits. Among them, the amylase activity in duodenum, jejunum, ileum of yarkand hares was extremely significantly higher than those of domestic rabbits（P ＜ 0.01）. The amylase activity got the maximum in jejunum of yarkand hares and domestic rabbits. 2) The cellulase activity in intestinal tract of yarkand hares was higher than those of domestic rabbits. Among them, the cellulase activity in cecum of yarkand hares was extremely significantly higher than those of domestic rabbits（P ＜ 0.01）, the cellulase activity in ileum of yarkand hares was significantly higher than those of domestic rabbits（P ＜ 0.05）. The cellulase activity got the maximum in cecum of yarkand hares and domestic rabbits. 3) The lipase activities in pancreas and intestinal tract of domestic rabbits were significantly higher than those of yarkand hares. Among them, the lipase activities in pancreas, duodenum, jejunum, ileum of domestic rabbits were significantly higher than those of yarkand hares（P ＜ 0.05）. Compared with the digestive tract, the lipase activity got the maximum in pancreas of domestic rabbits and yarkand hares, and the lipase activity of jejunum was the highest in intestinal tract. 4) The trypsin activity in pancreas and intestinal tract of domestic rabbits were higher than those of yarkand hares. Among them, the trypsin activity in duodenum and jejunum of domestic rabbits were significantly higher than those of yarkand hares（P ＜ 0.05）. The trypsin activity got the maximum in jejunum of domestic rabbits and yarkand hares. In conclusion, yarkand hares have strong abilities to digest starch and cellulose, but the digestion ability for fat was weak. The amylase and cellulase activities of yarkand hares were higher than those of domestic rabbits, and the lipase and trypsin activities of yarkand hares were lower than those of domestic rabbits.This could be one of the important factors that yarkand hares adapt to the barren and deficient food environment. |
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