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杨莎莎,徐志文,王承东,李才武,朱玲,周远成,李新琼,温安祥,李德生.2017.重组大熊猫IFN-γ聚氰基丙烯酸正丁酯纳米球的制备与评价.动物学杂志,52(2):314-321.
重组大熊猫IFN-γ聚氰基丙烯酸正丁酯纳米球的制备与评价
Preparation and Evaluation of Recombinant Interferon-gamma Nanosphere Loaded in Polybutylcyanoacrylate in Giant Panda (Ailuropoda melanoleuca)
投稿时间:2015-07-11  修订日期:2016-12-08
DOI:10.13859/j.cjz.201702015
中文关键词:  大熊猫  干扰素γ  聚氰基丙烯酸正丁酯  纳米球
英文关键词:Giant Panda, Ailuropoda melanoleuca  Interferon-gamma  Polybutylcyanoacrylate  Nanosphere
基金项目:四川省科技计划项目(No. 2012JQ0037)
作者单位E-mail
杨莎莎 四川农业大学生命科学学院 158335174@qq.com 
徐志文 四川农业大学  
王承东 中国保护大熊猫研究中心 雅安 625000  
李才武 中国保护大熊猫研究中心 雅安 625000  
朱玲 四川农业大学  
周远成 四川农业大学  
李新琼 四川农业大学  
温安祥 四川农业大学生命科学学院  
李德生 中国保护大熊猫研究中心 雅安 625000 1050133153@qq.com 
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中文摘要:
      为了制备并评价大熊猫(Ailuropoda melanoleuca)重组干扰素γ(IFN-γ)聚氰基丙烯酸正丁酯(PBCA)纳米球,本研究利用蛋白质原核表达技术,获得了重组大熊猫IFN-γ蛋白,然后以PBCA为载药材料,采用乳化聚合法制备了大熊猫干扰素γ纳米微球(IFNγ-PBCA-NS),最后借助感染大熊猫流感病毒A/Panda/Sichuan/01/2011(H1N1)的昆明小鼠(Mus musculus)模型,通过灌胃和皮下注射药物初步评价了IFNγ-PBCA-NS的药效。结果表明,大熊猫IFN-γ的蛋白分子量约为33.5 ku,所制备的大熊猫IFNγ-PBCA-NS外观规整,粒径在50 ~ 200 nm之间,跨距0.55,大小较均匀,包封率为56.7%,载药量为0.86%。灌胃和注射两种给药途径中,IFNγ-PBCA-NS组小鼠的生命延长率均显著高于IFN-γ组(P < 0.05或P < 0.01)。显示IFNγ-PBCA-NS在小鼠体内有更好的缓释和抗病毒作用,可为进一步研究制备大熊猫多肽类药物微粒提供参考。
英文摘要:
      To prepare and investigate the recombinant Interferon-gamma (IFN-γ) nanosphere loaded in polybutylcyanoacrylate (PBCA) in Giant Panda (Ailuropoda melanoleuca), we firstly obtained the recombinant IFN-γ protein through prokaryotic protein expression technology, and then, by choosing PBCA as the drug delivery material, prepared the INF-γ PBCA nanosphere (IFNγ-PBCA-NS) using an emulsion polymerization method. Further more, the Kunming Mice (Mus musculus), which were infected with giant panda influenza virus A/Panda/Sichuan/01/2011 (H1N1), were used as models to test the efficacy of IFNγ-PBCA-NS compared to that of IFNγ after intragastric administration and subcutaneous injection of drugs. The results demonstrated that the molecular weight of INF-γ protein was about 33.5 ku, and the IFNγ-PBCA-NS was globular in appearance, with uniform particle size. The diameters ranged from 50 to 200 nm, the span was 0.55, the encapsulation efficiency of the particles was 56.7% and the drug loading was 0.86%. Moreover, the prolonged life rate of mice in the IFNγ-PBCA-NS group was significantly higher than that of the IFNγ group in both intragastric administration and subcutaneous injection approaches (P < 0.05 or P < 0.01). In summary, IFNγ-PBCA-NS showed better sustained release and antiviral activity in mice, which could provide references for further research on the preparation of the Giant Panda peptide drug microparticles.
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