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曹建,王稳,李继荣,杨芳,王雪莲,郑思思,李来兴.2016.普通鸬鹚鸟卵溶菌酶与巢密度及卵顺序和新城疫病毒母源抗体关系.动物学杂志,51(2):310-318.
普通鸬鹚鸟卵溶菌酶与巢密度及卵顺序和新城疫病毒母源抗体关系
Lysozyme concentration in related to breeding density, laying order and Maternal Antibody of Newcastle Disease Virus in the Egg of Great Cormorant(Phalacrocorax Carbo)
投稿时间:2015-07-28  修订日期:2015-12-29
DOI:DOI: 10.13859/j.cjz.201602017
中文关键词:  普通鸬鹚  卵顺序  繁殖密度  溶菌酶  母源抗体  
英文关键词:Great Cormorant(Phalacrocorax carbo)  Laying order, Breeding density  Lysozyme, Maternal antibody
基金项目:国家重点基础研究发展计划(973计划);国家自然科学基金项目(面上项目)
作者单位E-mail
曹建 中国科学院西北高原生物研究所,青海西宁810008中国科学院高原适应与进化重点实验室青海西宁810008 中国科学院大学北京100049 caojianaymx@126.com 
王稳 中国科学院西北高原生物研究所,青海西宁810008 中国科学院高原适应与进化重点实验室青海西宁810008 中国科学院大学北京100049  
李继荣 中国科学院西北高原生物研究所,青海西宁810008 中国科学院高原适应与进化重点实验室青海西宁810008 中国科学院大学北京100049  
杨芳 中国科学院西北高原生物研究所,青海西宁810008 中国科学院高原适应与进化重点实验室青海西宁810008 ③中国科学院大学北京100049  
王雪莲 中国科学院西北高原生物研究所,青海西宁810008 中国科学院高原适应与进化重点实验室青海西宁810008 中国科学院大学北京100049  
郑思思 中国科学院西北高原生物研究所,青海西宁810008 中国科学院高原适应与进化重点实验室青海西宁810008中国科学院大学北京100049  
李来兴 中国科学院西北高原生物研究所,青海西宁810008 中国科学院高原适应与进化重点实验室青海西宁810008 中国科学院大学北京100049 lilx@nwipb.ac.cn 
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中文摘要:
      亲鸟可通过母体作用向鸟卵传递不同的免疫物质,用于免疫防御,对提高子代生存率至关重要。本研究在青海湖鸬鹚岛共采集60枚普通鸬鹚(Palacrocorax carbo)鸟卵,并同时记录巢密度和卵顺序,通过血凝抑制试验和溶解实验分别测定鸟卵溶菌酶浓度和新城疫病毒(Newcastle Disease Virus,NDV)母源抗体效价。对鸟卵溶菌酶浓度与巢密度进行利用Pearson 相关分析,利用单因素方差(One way ANOVA)分析鸟卵溶菌酶浓度与卵顺序关系,利用t检验分析新城疫病毒阳性鸟卵与阴性鸟卵溶菌酶浓度差异性,对新城疫病毒阳性鸟卵抗体效价与溶菌酶浓度进行Spearman相关分析。(1)不同窝的鸟卵溶菌酶浓度与巢密度呈正相关性(r = 0.886, P < 0.0001);(2)同一窝内鸟卵溶菌酶浓度随着卵顺序的增加而增加(F = 3.95, P< 0.05),且新城疫病毒阳性鸟卵的溶菌酶量高于阴性鸟卵(t = 4.08, df = 58, P < 0.001);(3)在新城疫病毒阳性鸟卵中,其新城疫病毒抗体效价与溶菌酶含量呈负相关关系(r =﹣0.840, P < 0.001)。上述结果表明,亲鸟会根据繁殖密度和卵顺序来调节向鸟卵分配的溶菌酶,同时会调节向鸟卵中传递抗新城疫病毒母源抗体和溶菌酶浓度,从而使免疫防御作用最大化。
英文摘要:
      Female birds can transmit immune factors into eggs to defense pathogen by maternal effect, including innate immune factor lysozyme and adaptive maternal antibody. To investigate how the female birds allocate immune factors into eggs , we totally collected 20 clutches and 60 eggs of great cormorant (Phalacrocorax carbo) in Qinghai Lake and recorded the breeding density and laying order, then determining Newcastle Disease Virus antibody titer by Hemagglutinin Inhibition Test and lysozyme concentration by lysoplate assay, respectively. The Pearson correlation and Spearman correlation were employed to analyze the correlation between lysozyme concentration and breeding density and the correlation between NDV antibody titer and lysozyme concentration respectively, meanwhile, the relationship between lysozyme level and laying order was performed by One way ANOVA and student’s t test was applied to analyze the difference of lysozyme concentration in NDV positive eggs and negative eggs. From the results, we found that (1) lysozyme concentration was positively related to breeding density between clutches (r = 0.886, P < 0.0001, Pearson correlation, Table 1); (2) lysozyme concentration increased with laying order within a clutch (F = 3.95, P < 0.05, Fig 1) and the level of lysozyme in the NDV positive eggs was higher than in negative eggs (t = 4.08, df = 58, P<0.001, Fig 2); (3) the NDV antibody titer had negative relationship with lysozyme concentration in positive eggs (r =﹣0.840, P < 0.001, Table 2). Hence, we concluded that female birds transmit lysozyme into eggs according to breeding density and laying order, meanwhile, effectively transferring lysozyme and NDV maternal antibody into eggs to provide optimal protection for offspring.
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