| 刘婷婷,袁静,彭静,俞诗源.2016.山丹黄参多糖对四氯化碳致小鼠急性肝损伤的影响.动物学杂志,51(4):583-589. |
| 山丹黄参多糖对四氯化碳致小鼠急性肝损伤的影响 |
| Effects of Shandan Sphallerocarpus racills Polysaccharide on Urgent Hepatic Injury Induced by CCl4 in Mice |
| 投稿时间：2015-08-17 修订日期：2016-07-06 |
| DOI：DOI: 10.13859/j.cjz.201604009 |
| 中文关键词: 山丹黄参多糖 四氯化碳 小鼠 肝组织 转化生长因子-β1 |
| 英文关键词:Shandan Sphallerocarpus gracilis polysaccharide carbon tetrachloride mice liver tissue TGF-β1 |
| 基金项目:甘肃省自然科学基金资助项目（No. 1107RJZA141） |
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| 中文摘要: |
| 为了研究山丹黄参多糖对四氯化碳（CCl4）致小鼠（Mus musculus）急性肝损伤及相关酶活性的影响，对50只小鼠分别灌胃0.2 mL山丹黄参多糖（12.5、25.0、37.5 g/L）或生理盐水7 d，最后一次灌胃1 h后腹腔注射1%的CCl4 橄榄油溶液0.2 mL，16 h后处死小鼠，比色法检测血浆丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）活力的变化，显微镜观察肝组织结构的变化，免疫组织化学法检测肝组织中转化生长因子-β1 (TGF-β1) 表达的变化。结果显示，与正常对照组相比，实验对照组（即灌胃生理盐水组）小鼠体重减轻，血浆丙氨酸氨基转移酶（ALT）和天门冬氨酸氨基转移酶（AST）活力显著升高（P < 0.01），肝明显肿胀，肝组织结构不清，肝细胞出现炎性坏死，转化生长因子-β1（TGF-β1）阳性表达明显增强（P < 0.01）。与实验对照组相比，山丹黄参多糖各组小鼠体重增加，血浆丙氨酸氨基转移酶（ALT）和天门冬氨酸氨基转移酶（AST）活力明显降低（P < 0.01），肝无明显肿胀，肝索清晰，炎性坏死很少，细胞结构清晰，转化生长因子-β1阳性表达明显减少（P < 0.01）。表明一定剂量的山丹黄参多糖能增强机体活力，促进机体新陈代谢，降低正常细胞的凋亡，对四氯化碳造成的肝组织损伤有一定的保护作用。 |
| 英文摘要: |
| To investigate the effect of Shandan Sphallerocarpus racills polysaccharide (SRP) on the urgent hepatic injury in mice (Mus musculus) induced by carbon tetrachloride (CCl4)，50 mice were gavaged with 0.2 ml SRP at different concentrations (12.5，25.0，37.5g/L) or physiological saline for 7 days，and intraperitoneally injected with 0.2 ml CCl4 solution (1% in olive oil) at the last day, the mice were sacrificed 16 h later. The morphological changes of hepatic tissues were observed under microscopy，the activities of plasma Alanine aminotransferase (ALT)，Aspartate aminotransferase (AST) were determined by colorimetry，the expression change of Transforming growth factor-β1(TGF-β1) in liver tissue were determined by immunohistochemical method．The dates were processing with spss 13.0. The results expressed in Mean ± SD. Results showed that contrast with the natural control group, the body weights are decreased in the experimental control group(Table 1), activities of plasma ALT，AST are significantly increased in the experimental control group（P < 0.01）（Table 2），the livers tissue structure are tumefaction clearly，liver cells are necrosis and seriously vacuolation in the experimental control group（Fig. 1），the positive expression of TGF-β1 in hepatic tissues are significantly increased (P< 0.01) in the experimental control group（Table 3）．However，contrast with the experimental control group, the body weights in the SRP groups are significantly increased（Table 1），the activity of plasma ALT，AST in the SRP groups are significantly decreased（P < 0.01）（Table 2），the liver have no obvious swelling，hepatic cord are clear，inflammatory necrosis are rarely，the liver celluar stucture are clear in the SRP groups（Fig. 1），the positive expression of TGF-β1 are decreased significantly（P < 0.01）in the SRP groups（Table 3）. Indicate that SRP may enhance the cellular activity，accelerate economy metabolism，and reduce cellular languish，protect the liver damage induced by carbon tetrachloride in mice． |
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