| 刘亚秋,李芳,赵健蓉,詹素平,王志坚.2016.大鳞副泥鳅仔稚鱼消化道黏液细胞分布及发育.动物学杂志,51(4):623-632. |
| 大鳞副泥鳅仔稚鱼消化道黏液细胞分布及发育 |
| Distribution and Development of Mucous Cells in Digestive Tract of Larvae and Juvenile in Loach (Paramisgurnus dabryanus) |
| 投稿时间：2015-09-28 修订日期：2016-05-06 |
| DOI：DOI: 10.13859/j.cjz.201604014 |
| 中文关键词: 大鳞副泥鳅 仔稚鱼 消化道 粘液细胞 发育 分布 |
| 英文关键词:Paramisgurnus dabryanus larva1 and juvenile digestive tract mucous cells development distribution |
| 基金项目:公益性行业(农业)科研专项(201203086) |
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| 中文摘要: |
| 利用组织学切片及阿利新蓝-过碘酸雪夫（AB-PAS）组化染色技术对0 ~ 50日龄大鳞副泥鳅（Paramisgurnus dabryanus）消化道各段黏液细胞的发育与分布进行显微观察和研究。大鳞副泥鳅黏液细胞分为Ⅰ、Ⅱ、Ⅲ和 Ⅳ4种类型。消化道黏液细胞最早出现在4日龄仔鱼的口咽腔和食道。10 ~ 15日龄口咽腔和食道黏液细胞数量快速增长，15 ~ 20日龄肠道各型黏液细胞数量显著增长，20日龄后消化道黏液细胞分布广泛。随着仔稚鱼发育，消化道各部黏液细胞主要以Ⅲ和 Ⅳ型细胞为主。根据大鳞副泥鳅仔稚鱼消化道黏液细胞的发育特点，5 ~ 10日龄和15 ~ 20日龄为其消化道功能发育的两个敏感时期，20日龄后消化道功能逐渐发育完善。建议加强对5 ~ 20日龄仔稚鱼的日常饲养管理以提高苗种成活率。 |
| 英文摘要: |
| Development and distribution of mucous cells in digestive tract of larval and juvenile (0﹣50 day-old) Paramisgurnus dabryanus was observed and analyzed by the method of histological sections and AB-PAS histochemical staining techniques. Three sections of larvae and juveniles digestive tract were chosen. Ten visions were random selected for measuring the number and size of mucous cells With Image-Pro Plus. All the measured dates were compared with one-way ANOVA using spss19.0. The level of significant difference was set at P < 0.05. The results indicated that there were four types of mucous cells (I, II, III, IV) in digestive tract of P. dabryanus (Fig 1). Mucous cells initially appeared in buccopharyngeal cavity and esophagus at 4 day-old. At 5 day-old, the type of mucous cells in buccopharyngeal cavity and esophagus was mainly II type, and the shape of them were globular or capsular (Fig 1a, b). Mucous cells initially appeared in anterior and middle intestine at 8 day-old, and type of them were I and III type (Fig 1c, d). The type of mucous cells in posterior intestine were mainly II type, and the shape of them were globular (Fig1e).There was a rapid increase of the number of mucous cells in buccopharyngeal cavity and esophagus between 10 and 15 day-old (Table 1). At 15 day-old, mucous cells were widespread in buccopharyngeal cavity and esophagus，and shape of them mainly was rod or globular (Fig 1f, g). The number of all type mucous cells in intestine increased significantly between 15 and 20 day-old (Table 1). Mucous cells were widespread in digestive tract after 20 day-old. Shape of most mucous cells in anterior and middle intestine were rod or capsular, while mucous cells’ shape in posterior intestine were globular or capsular at 20 day-old (Fig 1h ~ j). As the growth of larvae, mucous cells in digestive tract were mainly III and IV types. With larval and juvenile development, intestinal epithelial mucin PAS and AB (pH 2.5 and 1.0) reactions were gradually increased, indicating that the acidic and neutral mucilage of digestive tract secretion increased. And the AB (pH 2.5 and 1.0) reaction strength of anterior intestine and posterior intestine was higher than middle intestine (Table 2). Size of mucus cell in digestive tract is gradually increased. Larvae mucus cells in esophagus were largest in the same day-old, followed by Buccopharyngeal cavity, and were larger than mucous cells in the each part of intestinal (Table 3). At 5-15 day-old, secretion capability of mucus cell in esophagus was stronger than the other part of digestive tract (P < 0.05) (Fig 2). Secretion capability of mucus cell in anterior intestine increased significantly during 10-15 day-old (P < 0.05) (Fig 2). After 15 day-old, Secretion capability of mucus cell in posterior intestine increased significantly. In 20 day-old, secretion capability of mucus cell in middle intestine was weaker than anterior and posterior intestine (P < 0.05) (Fig 2).According to our researches, the sensitive periods of the development of larval and juvenile P. dabryanus were 5﹣10 day-old and 15﹣20 day-old, respectively. Digestive function gradually developed consummately after 20 day-old. In order to improvesurvival rate, it was necessary to strengthen larvae and juveniles daily feeding and management from 5 to 20 day-old. |
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