• 首页关于本刊期刊订阅编委会作者指南过刊浏览
徐绍刚,杨晓飞,田照辉,李文通,马峻峰.2016.硬头鳟与溪红点鲑杂交三倍体育种技术的初步研究.动物学杂志,51(5):887-894.
硬头鳟与溪红点鲑杂交三倍体育种技术的初步研究
Preliminary Study on Steelhead Trout (Salmon gairdneri) and Brook Trout (Salvelinus fontinalis) Hybrid Triploid Breeding Techniques
投稿时间:2015-10-22  修订日期:2016-04-18
DOI:DOI: 10.13859/j.cjz.201605019
中文关键词:  硬头鳟  溪红点鲑  杂交三倍体  6-二甲基氨基嘌呤  诱导
英文关键词:Rainbow  trout (Salmon  gairdneri) , Brook  trout (Salvelinus  fontinalis) , Hybrid  triploid, 6-dimethylaminopyridine (6-DMAP), Induction
基金项目:科技支撑项目(No. 2015BAD25B00),北京市农业局项目(No. SCSYZ201511-4),北京市财政项目(No. kjcx20140112),冷水性鱼类科技创新团队项目(No. JNKST201611)
作者单位E-mail
徐绍刚 国家淡水渔业工程技术研究中心 xushaogang@bjfishery.com 
杨晓飞 国家淡水渔业工程技术研究中心  
田照辉 国家淡水渔业工程技术研究中心 zh1973@126.com 
李文通 国家淡水渔业工程技术研究中心  
马峻峰 国家淡水渔业工程技术研究中心  
摘要点击次数: 843
全文下载次数: 810
中文摘要:
      为了获得同时具有杂交及三倍体优势的鲑鳟鱼苗种,开展了利用6-二甲基氨基嘌呤(6-DMAP)诱导硬头鳟(Oncorhynchus mykiss)、溪红点鲑(Salvelinus fontinalis)正反杂交受精卵的实验。杂交结果,溪红点鲑(♀)× 硬头鳟(♂)实验组卵子在受精10 min后全部死亡,而硬头鳟(♀)× 溪红点鲑(♂)组的受精卵正常,可以进行6-DMAP三倍体诱导实验。6-DMAP诱导受精卵结果,起始诱导时间在12 ~ 15 min,受精卵的发眼率最高,为75.87% ~ 76.64%,在5个起始诱导时间梯度内,受精卵的孵化率在86.44% ~ 90.31%之间,苗种三倍体率在92.45% ~ 95.55%之间,差异不显著(P > 0.05)。诱导持续时间为10 ~ 15 min时,受精卵发眼率达到最高,为74.28% ~ 76.81%,在5个诱导持续时间梯度内,受精卵孵化率在87.28% ~ 90.24%之间,苗种三倍体率在93.67% ~ 96.25%之间,变化不显著(P > 0.05)。药物诱导浓度为120 ~ 150 mg/L时,受精卵的发眼率达到最高,为73.57% ~ 76.27%,在5个不同的药物浓度梯度内,受精卵孵化率在88.57 % ~ 90.03%之间,苗种三倍体率在92.56% ~ 96.38%之间,变化不显著(P > 0.05)。结果表明,利用6-DMAP诱导受精卵制备杂交三倍体苗种的方法是可行的,实验最佳方法为硬头鳟(♀)× 溪红点鲑(♂)卵子受精后12 ~ 15 min内,利用浓度为120 ~ 150 mg/L的6-DMAP溶液诱导,持续时间为10 ~ 15 min,然后放入孵化桶内正常孵化,获得苗种的三倍体率可达92.56% ~ 96.38%,可满足正常生产的需要。
英文摘要:
      In order to obtain the salmon and trout seeds with the advantages of hybrid and triploid, steelhead trout (salmon gairdneri) and brook trout (Salvelinus fontinalis) reciprocal cross fertilized eggs of experimental induced by 6-dimethyl amino purine (6-DMAP) has been done. Hybridization results showed that the group eggs of brook trout (♀)×steelhead trout (♂) all died after 10 min after fertilization, and the development steelhead trout (♀)×brook charr (♂) fertilized eggs is normal, the triploid induction can be performed by 6-DMAP. With 6-DMAP in fertilized eggs result shows that the initial induction time for 12-15min, reached the highest eyed rate, 75.87%-76.64%, in the five initial induction time gradient groups, hatching rate between 86.44-90.31%, triploid rate between 92.45-95.55%, difference was not significant (P>0.05). The duration induced by 10-15min with 6-DMAP, reached the highest eyed rate 74.28-76.81%, in the five induced duration gradient groups, hatching rate between 87.28-90.24%, triploid rate between 93.67-96.25%, difference was not significant (P > 0.05). The fertilized eggs reach the highest eyed rate in induced concentration of 120-150 mg/L, 73.57-76.27%, in the five concentration gradient groups, the hatching rate between 88.57-90.03%, triploid rate between 92.56-96.38%, difference was not significant (P > 0.05).
附件
查看全文  查看/发表评论  下载PDF阅读器