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张婷婷,周立丽,李淑兰,赵丽.2016.三种内分泌细胞在极北鲵幼体胃内的发育.动物学杂志,51(5):853-860.
三种内分泌细胞在极北鲵幼体胃内的发育
The Development of Three Kinds of Endocrine Cells in Stomach of Larval Siberian Salamander (Salamandrella keyserlingii)
投稿时间:2015-10-25  修订日期:2016-07-10
DOI:DOI: 10.13859/j.cjz.201605015
中文关键词:  极北鲵    5-羟色胺细胞  胃泌素细胞  生长抑素细胞  幼体发育
英文关键词:Siberian Salamander (Salamandrella keyserlingii)  Stomach  5-hydroxytryptamine cells  Somatostatin cells  Gastrin cells  Larval development
基金项目:黑龙江省自然科学基金面上项目( No.C201219);
作者单位E-mail
张婷婷 哈尔滨师范大学生命科学与技术学院 1040689248@qq.com 
周立丽 哈尔滨师范大学生命科学与技术学院  
李淑兰 哈尔滨师范大学生命科学与技术学院 478970204@qq.com 
赵丽 哈尔滨师范大学生命科学与技术学院  
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中文摘要:
      采用免疫组织化学亲和素-生物素复合物(ABC)法研究极北鲵(Salamandrella keyserlingii)41至46期共6个时期幼体胃内5-羟色胺(5-HT)细胞、生长抑素(SS)细胞和胃泌素(GAS)细胞的分布位置及形态,5-羟色胺细胞和胃泌素细胞最先在41期幼体被检测出来,而生长抑素细胞发育略晚,在42期幼体才被检测到。文中采用Duncan多重比较的方法,对胃内同一种内分泌细胞不同发育时期的分布密度进行统计学分析,5-羟色胺细胞在41期分布最少,密度为1.2 ± 0.51,46期分布最多,密度为2.3 ± 0.91,随着胃的发育,5-羟色胺细胞密度呈递增的变化趋势;胃泌素细胞在42期分布最多,密度为2.1 ± 0.99,46期分布次之,密度1.7 ± 0.49,44期分布最少(密度1.0 ± 0.00),从42期到46期呈先减后增的变化趋势;而生长抑素细胞在43期密度(2.6 ± 0.99)高于其他各期,且差异显著(P < 0.05)。三种内分泌细胞最先均出现在胃上皮细胞之间,随着胃的不断发育,除了在上皮细胞之间分布以外,在腺泡上皮之间也有分布。不同内分泌细胞随着发育时间的不同,细胞形态也表现不同,如5-羟色胺细胞由最开始的椭圆形到后期的锥体形,胃泌素细胞由最先出现的锥体形到椭圆形再到锥体形,生长抑素细胞由锥体形到椭圆形。上述三种内分泌细胞的分布及形态学特点可能与极北鲵的生长发育以及胃的功能相适应。
英文摘要:
      The study was designed to explore the distribution and shape of the 5-hydroxytryptamine (5-HT) cells, the somatostatin (SS) cells and the gastrin (GAS) cells in the stomach of Siberian Salamander (Salamandrella keyserlingii) during the post-embryonic development (from the stages 41 to 46) by avidin-biotin complex methods (ABC). The results indicated that the 5-hydroxytryptamine cells and the gastrin positive cells were firstly found in the stomach at the 41st stage. Then the somatostatin positive cells were detected at the 42nd stage. Statistic analysis of the same endocrine cells at different stages to in the stomach showed that the 5-hydroxytryptamine cells were found the least at the stage 41 (1.2 ± 0.51) by Duncan methods, and the most at the stage 46 (2.3 ± 0.91). With the development of stomach of Siberian Salamander, the 5-hydroxytryptamine cells showed an increasing trend. The gastrin cells were most widely distributed at the stage 42 (2.1 ± 0.99), followed by the stage 46 (1.7 ± 0.49) and the distribution quantity was the least at the stage 44 (1.0 ± 0.00). Their distribution trend from the stages 42 to 46 was first decrease and later increase. The density distribution of the somatostatin cells was the highest at the stage 43 (2.6 ± 0.99) exceeding the rest stages, with significant difference (P < 0.05, Table 1). Three kinds of endocrine cells firstly appeared among the epithelium cells, and with the development of stomach they distributed among the acinar epithelial cells (PlateⅠ). The morphology of endocrine cells varied in different development stages, such as the shapes of 5-hydroxytryptamine cells were from the round to the cone, the shapes of gastrin cells were from the cone to round then to cone, and the shapes of somatostatin cells were from the cone to the ellipse. The distribution and shape characteristics of these endocrine cells may adapt to the growth and development of Siberian Salamander and the function of the stomach.
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