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崔弘,蔡善鑫,路丹,李鹤,彭双,国奥,李秀国.2016.人工诱导冬眠对中华蟾蜍血液和组织中宏量营养素的影响.动物学杂志,51(6):1018-1026.
人工诱导冬眠对中华蟾蜍血液和组织中宏量营养素的影响
Effects of Artificially Induced Hibernation on the Macronutrients in Blood and Tissues in Asiatic Toads, Bufo gargarizans
投稿时间:2015-11-12  修订日期:2016-08-21
DOI:DOI: 10.13859/j.cjz.201606010
中文关键词:  人工冬眠  血液  组织  宏量营养素  中华蟾蜍
英文关键词:Artificial hibernation  Blood  Tissue  Macronutrients  Asiatic Toads, Bufo gargarizans
基金项目:延边大学2016年大学生创新创业训练计划项目暨第八届本科生科研项目省级(校定)研究课题(No. ydbksky2016465);
作者单位E-mail
崔弘 延边大学医学院 吉林延吉 133002 cuihong@ybu.edu.cn 
蔡善鑫 延边大学医学院 吉林延吉 133002  
路丹 延边大学医学院 吉林延吉 133002  
李鹤 延边大学医学院 吉林延吉 133002  
彭双 延边大学医学院 吉林延吉 133002  
国奥 延边大学医学院 吉林延吉 133002  
李秀国 延边大学医学院 吉林延吉 133002 lixg@ybu.edu.cn 
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中文摘要:
      为探寻冬眠期间两栖动物血液和组织中宏量营养素的适应性改变过程,经人工诱导冬眠,检测了中华蟾蜍(Bufo gargarizans)在冬眠第1、3、7、14、28、42、56天的体重和脏器指数,以及血液、心、肝和骨骼肌组织中宏量营养素的含量。结果显示:1)冬眠期间中华蟾蜍体重未出现显著性变化,无性别差异。雄性蟾蜍的心、肝和腓肠肌的脏器指数显著性大于雌性(P < 0.01),但同一性别的脏器指数在冬眠期间无显著性变化。2)血中葡萄糖浓度自冬眠第42天起显著下降(P < 0.01);总蛋白在冬眠后第56天显著降低(P < 0.05),总胆固醇变化不显著。血中宏量营养素无性别差异。3)肝糖原自冬眠第42天起显著下降(P < 0.01),肌糖原自冬眠第1天起显著性下降(P < 0.05),而骨骼肌和心肌组织蛋白含量无显著变化。组织中宏量营养素无性别差异。人工诱导冬眠条件下,中华蟾蜍血液和组织中的糖类含量先迅速下降,血液中的蛋白成分只在深眠时才显著减少,但血液和组织中的宏量营养素水平可在1个月内维持稳定,这可能是其适应冬眠的主要生理学机制之一。
英文摘要:
      To investigate the effects of artificially induced hibernation on the consumption of macronutrients in the blood and tissues in Asiatic Toads, Bufo gargarizans, hibernation was artificially induced for 56 days, and the body mass, organic indexes of heart, liver and gastrocnemius muscle, as well as the concentrations of glucose, total protein and total cholesterol in blood, the contents of glycogen in the hepatic tissue and skeletal muscle, and protein in the skeletal muscle and myocardium were measured on the day before hibernation and the day of 1, 3, 7, 14, 28, 42 and 56 post hibernation. Results showed that, compared with the pre-hibernation, the body mass did not change significantly during hibernation, and there was not significant sexual difference (Plot a1 and a2 in Fig. 1). The organic indexes of the heart, liver and gastrocnemius muscle in male toads were significantly higher than those in females (P < 0.01) (Plot b2, c2 and d2 in Fig. 1), while both the males and females showed no significant change in organic indexes during hibernation (Plot b3, c3 and d3 in Fig. 1). The blood glucose concentration decreased significantly after day 42 (P < 0.01) (Plot a1 in Fig. 2), the concentration of total protein decreased significantly (P < 0.05) on day 56 (Plot b1 in Fig. 2), but total cholesterol did not change significantly (Plot c1 in Fig. 2). The blood macronutrients did not show significant sexual difference (Plot a2, b2 and c2 in Fig. 2). The hepatic glycogen decreased significantly after day 42 of hibernation (P < 0.01) (Plot a1 in Fig. 3), and the muscular glycogen decreased significantly after day 1 (P < 0.05) (Plot b1 in Fig. 3), but the protein contents in the skeletal muscle and myocardium did not show significant differences (Plot c1 and d1 in Fig. 3). The tissue macronutrients showed no significant sexual difference (Plot a2, b2, c2 and d2 in Fig. 3). The contents of carbohydrate in the blood and tissue decreased first, and the blood protein decreased significantly only after deep hibernation, but the macronutrients in the blood and tissue could keep a relatively stable state within 1 month. It might be one of the major physiological mechanisms to adapt to hibernation in toads.
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