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朱万龙,陈金龙,王政昆.2016.高山姬鼠下丘脑神经肽表达量的季节性变化.动物学杂志,51(5):817-825.
高山姬鼠下丘脑神经肽表达量的季节性变化
Seasonal Variations of Hypothalamic Neuropeptide Expression in Chevrier′s Field Mouse (Apodemus chevrieri)
投稿时间:2016-01-06  修订日期:2016-08-16
DOI:DOI: 10.13859/j.cjz.201605011
中文关键词:  高山姬鼠  体重调节  下丘脑神经肽
英文关键词:Chevrier′s Field Mouse, Apodemus chevrieri  Body mass regulation  Hypothalamus neuropeptide
基金项目:国家自然科学基金项目(No. 31260097;31560126),云南师范大学博士科研启动项目
作者单位E-mail
朱万龙 云南省高校西南山地生态系统动植物生态适应进化及保护重点实验室 zwl_8307@163.com 
陈金龙 昆明市海口林场  
王政昆 云南省高校西南山地生态系统动植物生态适应进化及保护重点实验室  
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中文摘要:
      为阐明高山姬鼠(Apodemus chevrieri)下丘脑神经肽表达量在季节性变化条件下对其体重调节的作用,测定了不同季节高山姬鼠的体重、体脂含量、食物摄入量以及血清瘦素浓度和神经肽Y(NPY)、刺鼠相关蛋白(AgRP)、阿片促黑色素原(POMC)和可卡因-安他非明转录调节肽(CART)表达量。采用食物平衡法测定高山姬鼠的食物摄入量,体脂含量用索氏抽提法进行测定,采用实时荧光PCR仪测定下丘脑神经肽表达量。采用单因素方差分析或协方差分析进行检验,相关性采用Pearson相关分析。高山姬鼠的体重和体脂均出现了季节性变化,冬季较低,夏季较高。食物摄入量季节性差异显著,冬季较高,夏季最低。血清瘦素含量也出现了季节性变化,与体脂变化趋势类似,瘦素含量与脂肪含量呈正相关关系。下丘脑神经肽NPY、AgRP、POMC和CART表达量季节性差异显著。食物摄入量与NPY和AgRP负相关,与POMC和CART正相关。以上结果表明,高山姬鼠在季节性变化过程中冬季降低体重、体脂,增加摄入量来维持生存。瘦素通过作用于下丘脑神经肽基因来调节高山姬鼠的体重平衡。
英文摘要:
      The aim of the present study was to examine the effects of hypothalamic neuropeptide genes expression on body mass regulation under seasonal variations in Chevrier′s Field Mouse (Apodemus chevrieri). Body mass, body fat mass, food intake, serum leptin levels and hypothalamic neuropeptide neuropeptide Y (NPY), agouti-related peptide (AgRP), pro-opiomelanocortin (POMC) and cocaine and amphetamine regulated transcript peptide (CART) expression were measured in Chevrier′s Field Mouse. Food intake was measured by food equity, body fat was extracted from the dried carcass by ether extraction in a Soxhlet apparatus, hypothalamic neuropeptides genes expression was measured by real-time q-PCR. Data were analyzed using One-way ANOVA or One-way ACNOVA, and associations were judged by Pearson-correlation analysis. The results showed that body mass and body fat mass showed seasonal variations, higher in summer and lower in winter (Fig. 1), and food intake also showed significant seasonal variability, higher in winter and lower in summer (Fig. 1). Serum leptin level also had similar seasonal variation, showing similar trend as body fat mass (Fig. 2), and serum leptin level showed a positive correlation with body fat mass (Fig. 3). Expression of hypothalamic neuropeptide NPY, AgRP, POMC and CART showed significant seasonal differences (Fig. 4). The above results suggest that Chevrier′s Field Mouse reduces body mass, body fat mass, increases food intake to survive in winter under seasonal changes. Leptin may play a regulatory role in body mass by acting on hypothalamic neuropeptide in Chevrier′s Field Mouse.
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