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高胜涛,苏岩,李芳,葛海龙,刘亚秋,王志坚.2017.嗜银细胞在菲牛蛭消化管中的形态与分布特征.动物学杂志,52(2):331-337.
嗜银细胞在菲牛蛭消化管中的形态与分布特征
Morphology and Distribution of Argyrophilic Cells in Digestive Tube of the Mexican Medical Leech (Poecilobdella manillensis)
投稿时间:2016-05-05  修订日期:2017-02-19
DOI:10.13859/j.cjz.201702017
中文关键词:  菲牛蛭  消化管  嗜银细胞  Grimelius银染法  形态
英文关键词:Mexican Medical Leech, Poecilobdella manillensis  Digestive tube  Argyrophilic cells  Grimelius' silver nitrate  Morphology
基金项目:重庆市万盛区科技局应用开发类项目(No. yykf-2014-002)
作者单位E-mail
高胜涛 西南大学 372825937@qq.com 
苏岩 西南大学  
李芳 西南大学  
葛海龙 西南大学  
刘亚秋 西南大学  
王志坚 西南大学 wangzj1969@126.com 
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中文摘要:
      运用Grimelius银染法对菲牛蛭(Poecilobdella manillensis)消化管内嗜银细胞的分布和形态进行观察,并根据嗜银细胞的分布特点统计其分布密度。消化管除食道外各部位均有嗜银细胞分布,细胞染棕色或黑色;嗜银细胞基本位于上皮细胞之间和固有层内,主要呈圆形、椭圆形、锥体形和长条形等多种形态;部分细胞胞突明显,细胞内可见黑色分泌颗粒。嗜银细胞分布密度为颚片最高,嗉囊次之,直肠最低,食道内未检测出嗜银细胞。消化管各段嗜银细胞的形态特征和分布规律可能与其食性和消化生理活动密切相关。
英文摘要:
      Grimelius' silver nitrate stain was used to investigate the morphology and distribution of argyrophilic cells in digestive tube of the Mexican Medical Leech (Poecilobdella manillensis). The distribution density of argyrophilic cells was counted according to the distribution characteristics. All the measured data were compared with Duncan multiple comparison using spss 17.0. The level of significant difference was set at P < 0.05. The results showed that argyrophilic cells did not exist in esophagus (PlateⅠ: 5), but distributed throughout most of the other portions in different densities (PlateⅠ). These cells were stained with brownish or black in the digestive tube. Argyrophilic cells in epithelium of lips had apophysis pointing to the oral cavity, and some of them had distinct cytoplasmic processes and inner cell visible black particles (PlateⅠ: 1). Argyrophilic cells were observed in the jaw, with shapes of circle, ellipse, strip and so on (PlateⅠ: 2, 3). Most argyrophilic cells in epithelium of crop and villus epithelium of intestine had apophysis pointing to the crop or intestine (PlateⅠ6﹣8), while a minority stretched their processes to the lamina porpria (PlateⅠ: 9), indicating the possible luminal secretion and endocrine functions of these cells. The density of argyrophilic cells in the jaw was the highest (117.63 ± 14.08), while that in the rectum was the lowest (2.63 ± 0.91). There was extremely significant difference between the two parts (P < 0.05). No argyrophilic cells were also observed in the esophagus (Table 1). Based on these results, we inferred that the morphology and distribution of argyrophilic cells in the digestive tube might be closely related to the feeding and digestive physiological activities of this leech.
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