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尹志伟,徐润林.2017.附藓蛭态轮虫分离法的比较与分离条件的探索.动物学杂志,52(4):708-716.
附藓蛭态轮虫分离法的比较与分离条件的探索
Comparative Studies on the Extraction Methods and Conditions of Moss-dwelling Bdelloidea Rotifers
投稿时间:2016-06-27  修订日期:2017-06-14
DOI:10.13859/j.cjz.201704022
中文关键词:  附藓动物  蛭态轮虫  分离技术
英文关键词:Moss-dwelling-animals  Bdelloidea rotifer  Extraction technology
基金项目:国家自然科学基金项目(No. 31030015)
作者单位E-mail
尹志伟 中山大学生命科学学院 广州 510275 sumbit@foxmail.com 
徐润林 中山大学生命科学学院 广州 510275 xurunlin_1960@yahoo.com 
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中文摘要:
      蛭态轮虫是附藓无脊椎动物中常见的类群,由于个体较小,观察难度大,尤其是对其分离困难,故对此类群的研究一直落后于其他轮虫类群。本文以文献报道附藓蛭态轮虫的分离方法为基础,设定了不同的实验组,比较了不同分离方法的分离效率,并探讨了不同分离条件对附藓蛭态轮虫分离效率的影响。结果显示:与简单的震荡复苏法相比,蔗糖震荡复苏法可分离出更多的蛭态轮虫,且在分离条件为pH 7.8、温度20℃和湿度60%时可达到最高的分离效率。文中还就各分离条件对分离效率的影响进行了讨论。
英文摘要:
      Bdelloidea rotifers are the common invertebrates in moss fauna. However, Bdelloidea rotifers is difficulty to observe due to the smaller size of each in individual and also it is difficulty to separate them. Therefore the research on this group invertebrate was behind compared to other rotifer taxa. This article is aimed at comparing the extraction efficiency on Bdelloidea rotifers by different extraction methods and exploring the affect on extraction efficiency under different experiment condition. The multi-shock resuscitation methods and the sucrose shock resuscitation methods were selected according to the literature reports on extraction methods of moss-dwelling Bdelloidea rotifer. Impact factors, such as pH, temperature and humidity were chosen as different experimental conditions, and the experiment was done by setting up the different experimental groups with Orthogonal table of L9 (Table 1). All the data obtained from the experiment were deal with Excel 2010 and the statistic analysis was processed with SPSS 19.0 for analysis of variance to determine the difference among the experiment results. The study results showed that the average number of Bdelloidea rotifers were 94.68 ind/g and 165.33 ind/g by multi-shock and sucrose shock resuscitation methods, respectively (Fig.1). The sucrose shock resuscitation methods could extract more Bdelloidea rotifers compared with the multi-shock resuscitation methods significantly (F = 24.017, P< 0.05). The influence of the extraction conditions on the extraction efficiency was in the order: pH > humidity > temperature. Both range analysis and variance analysis displayed that while the maximum extraction efficiency can be expected under the following extraction conditions: pH at 7.8, temperature at 20℃ and humidity at 60% respectively (Table 2﹣4).
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