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余陆伟,张梦茹,刘晨辉,王言豪,刘志伟.2018.黄扯旗鱼核型分析方法及核型特征.动物学杂志,53(1):99-105.
黄扯旗鱼核型分析方法及核型特征
Karyotyping the Pristella Tetra (Pristella maxillaris)
投稿时间:2017-02-07  修订日期:2017-10-29
DOI:10.13859/j.cjz.201801013
中文关键词:  黄扯旗鱼  核型分析  染色体
英文关键词:Pristella Tetra (Pristella maxillaris)  Karyotype analysis  Chromosome
基金项目:上海市教委创新项目(No. 13YZ094),上海海洋大学大学生创新计划项目(No. xj2013011)
作者单位E-mail
余陆伟 上海海洋大学 m160101090@st.shou.edu.cn 
张梦茹 上海海洋大学 1413321@st.shou.edu.cn 
刘晨辉 上海海洋大学 1413525@st.shou.edu.cn 
王言豪 上海海洋大学  
刘志伟 上海海洋大学 zwliu@shou.edu.cn 
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中文摘要:
      本研究的目的在于建立黄扯旗鱼(Pristella maxillaris)的核型分析方法,以了解该物种的染色体数目、形态及分类等遗传学特征。比较头肾-PHA法和胚胎制片法制备黄扯旗鱼染色体样品的异同。结果发现,头肾-PHA法难度较大,操作复杂且制备的分裂相细胞数目较少;而胚胎法操作简单,用时短,制备的分裂相细胞稳定且数目多。随后基于胚胎制片法利用Photoshop CS5和image J软件进行了黄扯旗鱼的核型分析。统计结果表明,黄扯旗鱼核型为2n = 52 = 6m + 12sm + 34t,NF = 70,且未发现多倍体、异型性染色体及随体染色体的现象。
英文摘要:
      This study aimed to test a convenient and effective method for chromosome preparation and karyotyping in Pristella Tetra (Pristella maxillaris). The chromosome samples made from an embryo were compared with those made from the pronephros-PHA method. Then karyotype analysis was conducted by Photoshop CS5 and image J. We found that pronephros-PHA method was hard to manipulate, due to twice injections, dissection of pronephros in small fish, and less good chromosome samples. While embryo method was easy to manipulate, and steadily produced more good chromosome samples (Fig. 1). The results showed that the diploid chromosome number was 2n = 52 and the karyotype was composed of 6m + 12sm + 34t, NF = 70 (Fig. 2 and Table 1). No chromosome polyploidy, heteromorphic sex chromosome, and randon chromosome segregation were found.
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