33 bat specimens were collected during the field survey from July to September 2015 in Xingren County, Pingba County, and Xiawutun Town, Jingnan Town of Xingyi City of Guizhou Province and kept in the Animal Specimens Room, School of Life Sciences, Guizhou Normal University, China. These specimens were identified as Myotis siligorensis (which is new to Guizhou Province) based on morphological evidences and molecular phylogenic analyses. The main taxonomy characteristics are listed as follow: small body size, the forearm length is 36.03 ± 1.50 mm (32.66﹣38.98 mm, n = 33); long and narrow ears; straight and slender tragus; third metacarpal length is longest, fifth metacarpal length is shortest; Penile length is 4.52 ± 0.84 (2.85﹣5.75 mm, n = 21); narrow and prominence skull; the greatest length of skull is 13.87 ± 0.74 (13.00﹣14.88 mm, n = 8), the brain-case height is 6.36 ± 0.24 (6.03﹣6.74 mm, n = 8); smaller auditory bullae; sagittal crest thin and weak; the maxillary 1 and 2 incisors inclined to the central, the first maxillary incisor has a main cusp and 1 attached tip; second incisor smaller than the first incisors, separated from canine by small diastema; smaller premolar (P3) was located in the tooth row. The data of morphology and skull measurements consistent with M. siligorensis. In the Bayesian phylogenetic tree, the collected specimens formed a cluster with M. siligorensis with posterior rate 99%. The molecular phylogenic analysis furtherly proved that these specimens are M. siligorensis, and the genetic distance is 0.03 only. It seemed that the species of M. siligorensis have a continuous distribution in Southeast Asia.