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张亚男,熊建利,刘强强,闵遥,苟健平,李俊蓉.2018.龙洞山溪鲵的血细胞组成及血红蛋白含量检测.动物学杂志,53(1):75-81.
龙洞山溪鲵的血细胞组成及血红蛋白含量检测
Blood Cell Composition and Hemoglobin Concentration in Batrachuperus londongensis
投稿时间:2017-03-31  修订日期:2017-12-29
DOI:10.13859/j.cjz.201801010
中文关键词:  血细胞  血液参数  小鲵科  山溪鲵属  两性差异
英文关键词:Blood cells  Hematological parameters  Hynobiidae  Batrachuperus  Sexual difference
基金项目:国家自然科学基金项目(No. 31471971,30900138)和河南省高等学校青年骨干教师资助计划项目(No. 2015GGJS-054)
作者单位E-mail
张亚男 河南科技大学动物科技学院 1126360027@qq.com 
熊建利 河南科技大学动物科技学院 xjlpanda@126.com 
刘强强 河南科技大学动物科技学院 1195549782@qq.com 
闵遥 河南科技大学动物科技学院 630967440@qq.com 
苟健平 河南科技大学动物科技学院 1911748216@qq.com 
李俊蓉 河南科技大学动物科技学院 734026927@qq.com 
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中文摘要:
      血液学指标不仅可以反映动物生理和病理变化,也能体现动物对环境的适应。本文利用血液学方法检测了27尾龙洞山溪鲵(Batrachuperus londongensis)个体的血细胞组成和血红蛋白含量,龙洞山溪鲵血红蛋白平均含量为4.16 × 10-2 g/ml;红细胞卵圆形,细胞核椭圆形位于中央或亚中央,红细胞平均含量为6.04 × 104 个/mm3;白细胞数量较少,多为圆形或近圆形,平均含量为2.90 × 103 个/mm3;白细胞中淋巴细胞最多,其次为单核细胞、嗜碱性细胞和中性细胞,嗜酸性细胞最少。血液参数中仅中性细胞和嗜碱性细胞的百分比在雌雄之间存在显著差异;头体长、体重和各血液参数的相关性均不显著。同时,将研究结果与其他小鲵科动物的数据进行了比较。本文报道的龙洞山溪鲵血细胞组成和血红蛋白含量的基础数据为其后续的健康监测和诊断以及适应进化的研究提供了基础资料。
英文摘要:
      Study on the hematology can not only help to understand the physiological and pathological variations of animals, but also detect the adaptation of animals to the environment. However, studies on the hematology of amphibians, especially in the Urodela, are limited. Here, the blood cell composition and hemoglobin concentration of Batrachuperus londongensis were researched in 27 individuals. Comparison between sexes was conducted by Independent-Samples T Test or Mann-Whitney U, and correlations between snout-vent length and body mass (Table 1) and hematological parameters were determined by linear regression. All statistical tests were performed with SPSS software. The mean value of hemoglobin concentration was 4.16 × 10-2 g/ml; erythrocytes were oval with ellipsoidal nucleus that was located in the center or eccentric position of the cell (Fig. 1), and mean erythrocyte count was 6.04 × 104 cell/mm3; the number of leucocytes was small, and its mean count was 2.90 × 103 cell/mm3 (Table 2). The lymphocytes were richest leucocytes, followed by monocytes, basophils, neutrophils, and eosinophils (Table 2). Among the hematological parameters, only the percentage of neutrophils and basophils showed significant sexual difference. Correlations between snout-vent length, body mass and hematological parameters were not significant (Table 3). Meanwhile, comparison between our results with those of other hynobiid salamanders was carried out. These basic data of blood cell component and hemoglobin concentration reported in this study are useful in the health monitoring and diagnosis, and in understanding the evolution and adaptation of B. londongensis.
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