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薛玉环,唐鸣慧,胡巍堃,金鑫,张艺钟,王晓丽.2018.奥尼罗非鱼肠道杯状细胞分布及分型观察.动物学杂志,53(3):456-460.
奥尼罗非鱼肠道杯状细胞分布及分型观察
Distribution and Classification of Intestinal Goblet Cells in Tilapia Galilaea
投稿时间:2017-07-13  修订日期:2018-04-23
DOI:10.13859/j.cjz.201803013
中文关键词:  奥尼罗非鱼  肠道  杯状细胞  分型
英文关键词:Tilapia Galilaea, Oreochromis niloticus ♀ × Oreochromis aureus ♂  The intestinal tract  Goblet cells  Classification
基金项目:
作者单位E-mail
薛玉环 广西大学动物科学技术学院 410989990@qq.com 
唐鸣慧 广西大学动物科学技术学院 775698076@qq.com 
胡巍堃 广西梧州市万秀区龙湖镇水产畜牧兽医站 327682010@qq.com 
金鑫 广西大学动物科学技术学院 2365997760@qq.com 
张艺钟 广西大学动物科学技术学院 342258648@qq.com 
王晓丽 广西大学动物科学技术学院 xiaoliwang@gxu.edu.cn 
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中文摘要:
      本研究利用石蜡切片结合阿利新蓝-高碘酸Schiff(AB-PAS)和醛品红-阿利新蓝(AF-AB)染色法研究奥尼罗非鱼(Oreochromis niloticus ♀ × Oreochromis aureus ♂)肠道内杯状细胞的分布与分型。结果发现,奥尼罗非鱼肠道黏膜层较厚,有大量肠绒毛突起,肠绒毛的数量从前肠到后肠逐渐减少。杯状细胞主要位于肠绒毛上皮细胞间,形状大多为椭圆或近似圆形,也有呈细长梭形。经统计分析可知,肠道黏膜上皮单位面积中杯状细胞数量从前肠到后肠逐渐增多,前肠、中肠、后肠杯状细胞平均数量分别为(230.0 ± 4.9)个/ mm2、(287.9 ± 7.5)和(336.7 ± 10.0)个/ mm2,具有较大差异,三者间两两相比差异显著(P < 0.05)。奥尼罗非鱼肠道杯状细胞以中性-酸性双染为主,酸性杯状细胞在肠道各段所占比例较低,且以硫酸化亚型杯状细胞为主,缺少羧酸化亚型杯状细胞。
英文摘要:
      The distribution and types of goblet cells in the intestine of Tilapia Galilaea (Oreochromis niloticus ♀ × Oreochromis aureus ♂) were studied by Alcian Blue-Periodic Acid Sthiff (AB-PAS) and Aldehyde Fuchsin-Alcian Blue (AF-AB) staining. The results showed that the intestinal mucosal layer of tilapia was thick, and a large number of intestinal villi decreased gradually from foregut to hindgut. Goblet cells were mainly located between the epithelial cells of intestinal villi, and their shape was mostly oval or approximately circular, and some were in a shape of a long and thin spindle (Fig. 1). The statistical analysis showed that the number of goblet cells per unit area from foregut to hindgut gradually increased, the average numbers of goblet cells in foregut, midgut and hindgut of were 230.0 ± 4.9 cell/mm2, 287.9 ± 7.5 cell/mm2 and 336.7 ± 10.0 cell/mm2, respectively, with significant difference among the three groups (P < 0.05, Table 1). PAS and AB-PAS staining showed that the goblet cells in tilapia intestinal were mainly neutral and acid double staining, the acid goblet cells were low in the intestinal segments (Fig. 2, 3), and the sulfuric acid cells were mainly sulfuric acid cells in AF-AB staining (Fig. 3), and the cups of goblet cells were lack of carboxylic acid.
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