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杨杨,宋小晶,唐文乔,张亚.2018.克氏光唇鱼线粒体基因组测定及光唇鱼属的系统发育分析.动物学杂志,53(2):207-219.
克氏光唇鱼线粒体基因组测定及光唇鱼属的系统发育分析
Complete Mitochondrial Genome of Acrossocheilus kreyenbergii, with Phylogenetic Analysis of Genus Acrossocheilus
投稿时间:2017-07-19  修订日期:2018-03-02
DOI:10.13859/j.cjz.201802006
中文关键词:  克氏光唇鱼  线粒体基因组  系统发育  光唇鱼属
英文关键词:Acrossocheilus kreyenbergii  Mitochondrial genome  Phylogenetic analysis  Acrossocheilus
基金项目:国家科技基础性工作专项(No. 2015FY110200)
作者单位E-mail
杨杨 海洋动物系统分类与进化上海高校重点实验室 yyangyangy0308@163.com 
宋小晶 海洋动物系统分类与进化上海高校重点实验室 xiaojingsong23@163.com 
唐文乔 海洋动物系统分类与进化上海高校重点实验室 wqtang@shou.edu.cn 
张亚 海洋动物系统分类与进化上海高校重点实验室 zhangya@shou.edu.cn 
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中文摘要:
      根据侧条光唇鱼(Acrossocheilus parallens)线粒体基因(mtDNA)序列设计引物,采用引物步移和PCR扩增产物测序,获得了克氏光唇鱼(A. kreyenbergii)的mtDNA全序列。结构分析表明,克氏光唇鱼mtDNA为首尾闭合的环状基因,全长16 596 bp,编码37个基因,包括13个蛋白编码基因、22个tRNA基因、2个rRNA基因和两段非编码区(D-loop和轻链复制起点OL),碱基组成具有明显的A + T偏好和反G偏倚现象。13个蛋白编码基因中,除COⅠ的起始密码子是GTG,其余基因的起始密码子均为ATG;终止密码子包括完全的终止密码子TAA(38.46%)和TAG(7.69%),不完全的终止密码子TA(15.38%)和T(38.46%)。在D-loop区的811 ~ 837 bp区间发现了一段“TA”短串联重复序列。从蛋白编码基因所包含的信息量、变异位点和变异率看,ND5、ND4、COⅠ和ND2最适合作为光唇鱼属种间系统发育分析的分子标记。采用贝叶斯法利用13个蛋白编码基因所构建的系统发育树显示,光唇鱼属和白甲鱼属(Onychostoma)的24种鱼类各自没有聚为单系群,相互间不能明确区分。
英文摘要:
      According to the existing mitochondrial gene sequence of Acrossocheilus parallens, primers were designed, and by using the method of primer walking and PCR amplification, mitochondrial genome sequence of A. kreyenbergii was obtained, and its structure was analyzed. The results showed that the complete mitochondrial genome of A. kreyenbergii was a circular genome, 16 596 bp in length, including 13 protein coding genes, 22 tRNA genes, 2 rRNA genes and two non-coding regions (D-loop and origin of light strand replication, OL). The base composition showed two features, the negative bias for G and AT bias. All protein-coding genes started with ATG as an initiation codon except COⅠ, which used GTG. Two complete termination codons were TAA (38.46%) and TAG (7.69%), and two incomplete stop codons were TA (15.38%) and T (38.64%). A control region of 941 bp in length between tRNAPro and tRNAPhe, a short tandem repeat TA, was found in the 811﹣837 bp intervals. From the information contained in the protein coding gene, the mutation site and the mutation rate of view, ND5, ND4, COⅠ and ND2 were the most suitable molecular markers for interspecific phylogenetic analysis of Acrossocheilus. The phylogenetic tree constructed by Bayesian method for 13 protein coding genes showed that 23 species of Acrossocheilus and Onychostoma were not clustered into monophyletic groups, and could not be clearly distinguished from each other.
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