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王守红,李豪,刘露莎,张兴其,江建平.2018.温度对饰纹姬蛙蝌蚪生长的影响.动物学杂志,53(2):191-197.
温度对饰纹姬蛙蝌蚪生长的影响
Effect of Temperature on Growth of Microhyla fissipes Tadpole
投稿时间:2017-09-04  修订日期:2018-03-05
DOI:10.13859/j.cjz.201802004
中文关键词:  饰纹姬蛙  蝌蚪  生长  温度
英文关键词:Microhyla fissipes  Tadpoles  Growth  Temperature
基金项目:中国科学院重点部署项目(No. KJZD-EW-L13),国家自然科学基金项目(No. 31471964)
作者单位E-mail
王守红 中国科学院成都生物研究所 wangsh@cib.ac.cn 
李豪 四川师范大学生命科学学院 1141900647@qq.com 
刘露莎 中国科学院成都生物研究所 liuls@cib.ac.cn 
张兴其 四川师范大学生命科学学院 1246004047@qq.com 
江建平 中国科学院成都生物研究所 jiangjp@cib.ac.cn 
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中文摘要:
      为揭示温度对饰纹姬蛙(Microhyla fissipes)蝌蚪生长的影响,将同一家系发育至28期的45只蝌蚪随机均分为3组,每组15只,分别置于水温25、28、30 ℃条件下饲养,测定其全长、体宽、眼距、吻长及头体长5项形态指标,分析不同温度下各项形态指标的变化。结果显示,5项形态特征有基本一致的变化趋势,其中全长最易表征蝌蚪的生长状况。从全长看,25 ℃组蝌蚪生长发育缓慢,但在12 d后有加快的趋势;30 ℃组在饲养第3 ~ 12 d,生长最迅速(增长率β = 50.05%),但在12 d后生长明显变缓(β = 9.42%),而到28 d时,已有较多蝌蚪死亡(5/15);28 ℃组的蝌蚪生长发育态势最好,且在饲养第28 d均超过30 ℃组,其在3 d后加快的生长趋势也强于25 ℃组。其他4项指标与全长有类似的结果。综合结果分析表明,30 ℃下蝌蚪在后期(21 d后)的成活率明显降低,而25 ℃下其生长缓慢,提示28 ℃是饰纹姬蛙蝌蚪比较适合的饲养温度。
英文摘要:
      Environment factors affect many larval amphibian traits, including larval growth, development, and time to metamorphosis. Temperature is found to be a key factor in the growth larval among all the environment factors. To uncover the effect of temperature on tadpole growth of Microhyla fissipes, adults M. fissipes collected from Shuangliu, Chengdu, China (30°34′57″N, 103°50′38″E) were used in this work and induced breeding with Luteinizing Hormone Releasing Hormone analogue hormones (LHRHa, dosage: 0.3 μg/g body weight) in laboratory. Forty-five full-sib family tadpoles of stage 28 (stages according to Wang et al. 2017) were randomly and averagely divided into three groups and raised at water temperature (WT) 25 ℃, 28 ℃ and 30 ℃, respectively. Five morphological measurements (Fig. 1) including total length (TOL), body width (BW), interocular space (IOS), snout length (SL) and snout-vent length (SVL) were measured by a stereo microscope with Mshot Image Analysis system (Mc50-N). We used one-way ANOVA and DUNCAN test to analyze the data by the sigmaplot 13.0 software. The growth rate β (%) = (the ending data-beginning data) / beginning data × 100%. Descriptive statistics was presented as mean ± standard error, and the significance level was set at P < 0.05. The results showed that the five measured morphological characters had similar growth trend, of which TOL could be the dominant character to indicate the growth of tadpoles (Fig. 2). The TOL indicated that tadpoles under 25 ℃ grew slowest but presented quickening growth trend after 21 d; tadpoles under 30 ℃ grew fastest but presented slower growth trend after 12 d, and 5 tadpoles were died before end of feeding 28 d; tadpoles under 28 ℃ grew continuously fast and the TOL is longer than those under 30 ℃ at 28 d, also presented quickening growth trend after 21 d, which higher than that under 25 ℃ (Table 1). So, it could be concluded that the survival ratio of tadpoles under 30 ℃ decreased, tadpoles grow slower under 25 ℃, and 28 ℃ is the best feeding temperature for tadpoles of M. fissipes.
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