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谢木娇,钟金香,颉晓勇,李俊伟,朱长波.2018.圆尾鲎消化道组织结构与黏液细胞分布.动物学杂志,53(2):270-277.
圆尾鲎消化道组织结构与黏液细胞分布
Histology and Mucous Cell Distribution in Digestive Tract of Carcinoscorpius rotundicauda
投稿时间:2017-09-13  修订日期:2018-03-12
DOI:10.13859/j.cjz.201802013
中文关键词:  圆尾鲎  消化道  组织学  黏液细胞
英文关键词:Carcinoscorpius rotundicauda  Digestive tract  Histology  Mucous cell
基金项目:广东省海洋渔业科技推广项目(No. B201601-01),广东省省级科技计划项目(No. 2013B090500081,2013B040500020)
作者单位E-mail
谢木娇 中国水产科学研究院南海水产研究所 zuolan521314@126.com 
钟金香 广东省海洋与渔业技术推广总站  
颉晓勇 中国水产科学研究院南海水产研究所 xiexiaoyongsh@sina.com 
李俊伟 中国水产科学研究院南海水产研究所农业部南海渔业资源开发利用重点实验室广东省渔业生态环境重点实验室  
朱长波 中国水产科学研究院南海水产研究所农业部南海渔业资源开发利用重点实验室广东省渔业生态环境重点实验室  
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中文摘要:
      利用石蜡切片、苏木素-伊红(H.E)和阿利新兰-高碘酸雪夫试剂(AB-PAS,AB,pH 2.5)组化染色技术研究圆尾鲎(Carcinoscorpius rotundicauda)消化道组织结构及其黏液细胞的分布特征。圆尾鲎消化道外形为一直管状,中肠部位有分支。H.E染色结果显示,食道、胃、幽门、中肠与后肠的管壁一般结构由外至内分为外膜、肌肉层、黏膜下层、黏膜层。食道是一段短管状结构,肌肉层较胃壁的薄但几丁质层极厚。胃则为一膨大的砂囊结构,内含一定数量的纵行黏膜皱褶,肌肉纤维排列整齐,几丁质层较薄。幽门与中肠套叠,幽门壁肌肉层很薄,几丁质层清晰可见。中肠和后肠结构差异不大,具有一定数量的黏膜皱褶,上皮细胞间分布比较多的黏液细胞,均无几丁质层。AB-PAS组化染色结果显示,消化道有Ⅰ和Ⅱ型两种黏液细胞,不同部位分布数量差别很大。食道和幽门未见黏液细胞。胃黏膜下层有少量Ⅱ型黏液细胞。中肠和后肠黏液细胞数量比较多,尤其是与幽门套叠的中肠前端区域,均以Ⅱ型黏液细胞为主,主要分布在黏膜下层和黏膜上皮。在后肠黏膜下层有Ⅰ型黏液细胞分布,而黏膜上皮则分布密集的Ⅱ型黏液细胞。圆尾鲎消化道组织结构及黏液细胞分布特征反映其不同部位功能的差别,体现食性与消化机能相协调的特点。
英文摘要:
      This work was conducted to study the histological properties and distribution of mucous cells in digestive tract by conventional paraffin section, H.E and AB-PAS (Alician blue and periodic acid Schiff reagent, Alician blue at pH 2.5) staining in Carcinoscorpius rotundicauda. The digestive tract shaped as a tube with branch structure in the zone of midgut (Fig. 1). H.E staining showed that esophagus, stomach, pyloric stomach, midgut and hindgut of this species showed general structure of digestive tract. From inner to outer of the digestive tube were mucosa, submucosa, muscularis, and serosa layer. A short and tubular esophagus was featured by thin muscularis but thick deeply chitin layer (Fig. 2a, b); Stomach was expanded with thick longitudinal folds and chitin layer coating in the mucosa surface (Fig. 2c, d, e). There pyloric stomachs trapped into midgut with both thin but recognizable muscularis and chitin layer (Fig. 2f, g); there was no remarkable difference in structure between midgut and hindgut, they both had numbers of mucosa fold and mucous cells locating in epithelium, but there was no chitin layer (Fig. 2h﹣l). Results of AB-PAS staining showed that there existed typeⅠ and typeⅡ mucous cells in digestive tract and their distribution characteristics were different in number and shape through the digestive tract. No mucous cell was detected in esophagus and pyloric stomach, but typeⅡ mucous cells were found in submucosa layer of stomach (Fig. 3a). Mucous cells distributed in mudgut and hindgut were superior in quantity than in stomach to some extent, especially in gut zone near pyloric stomach. The majority of mucous cells was TypeⅡ in midgut, mainly distributed in submucosa layer and mucosa epithelium (Fig. 3b, c). TypeⅠ mucous cells were found in submucosa layer and numerous typeⅡ mucous cells were found in mucosa epithelium of hindgut (Fig. 3d﹣f). The histological characteristics of digestive tract of C. roundicauda reveal that the functions may differ in different parts of digestive tract, and it may indicate a coordination between feeding habit and digestive tract structural evolution in C. rotundicauda.
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