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梁娟娟,杨明,任敏,汪焕,张盛周,吴孝兵.2018.性类固醇激素受体ER、AR和PR在牛蛙胃肠胰的定位.动物学杂志,53(3):446-455.
性类固醇激素受体ER、AR和PR在牛蛙胃肠胰的定位
Expression of Sex-steroid Hormone Receptors ER, AR and PR in the Gastro-entero-pancreatic System of Bullfrog, Rana catesbeiana
投稿时间:2017-10-17  修订日期:2018-04-26
DOI:10.13859/j.cjz.201802101
中文关键词:  牛蛙  胃肠胰  性类固醇激素受体  免疫组织化学
英文关键词:Bullfrog, Rana catesbeiana  Gastro-entero-pancreatic system  Sex-steroid hormone receptor  Immunohistochemistry
基金项目:安徽省自然科学基金项目(No. 11040606M75)
作者单位E-mail
梁娟娟 安徽师范大学生命科学学院 394348424@qq.com 
杨明 安徽师范大学生命科学学院 791816538@qq.com 
任敏 安徽师范大学生命科学学院 690176907@qq.com 
汪焕 安徽师范大学生命科学学院 1063724321@qq.com 
张盛周 安徽师范大学生命科学学院 szzhang@mail.ahnu.edu.cn 
吴孝兵 安徽师范大学生命科学学院 wuxb@mail.ahnu.edu.cn 
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中文摘要:
      本文用免疫组织化学技术检测及光密度定量分析,研究4种性类固醇激素受体在牛蛙(Rana catesbeiana)胃肠胰内的定位及表达的强弱,探讨4种性类固醇激素在牛蛙胃肠胰中的功能。染色结果显示,4种性类固醇激素受体在牛蛙胃肠胰内都有分布,雌雄之间分布差异较小。雌激素受体α(ERα)主要分布在牛蛙胃腺、直肠固有层和胰腺中;雌激素受体β(ERβ)主要分布在食道上皮、直肠固有层和胰腺中;雄激素受体(AR)主要分布在食道上皮、直肠固有层和胰腺中;孕激素受体(PR)主要分布在空肠、食道上皮和胰腺中。光密度检测结果显示,雌激素受体α(ERα)在牛蛙胃和直肠中阳性反应最强,在胰腺中相对较弱。雌激素受体β(ERβ)在牛蛙直肠中阳性反应最强,胰腺和食道次之。雄激素受体(AR)在食道中阳性反应相对较强,其他部位都较弱。孕激素受体(PR)在空肠中的阳性反应较强,其他部位都较弱。雌激素受体α(ERα)在胃中以及雄激素受体(AR)在食道和直肠中的免疫阳性反应均是雌性牛蛙强于雄性牛蛙,雌激素受体β(ERβ)在胰腺中的免疫阳性反应是雄性牛蛙强于雌性牛蛙。4种性类固醇激素受体中,雌激素受体α(ERα)和雌激素受体β(ERβ)在牛蛙胃肠胰中的分布最多,雄激素受体(AR)、孕激素受体(PR)的分布相对较少。性类固醇激素受体主要分布在食道、胃、直肠和胰腺中,其中,分布最多的部位是胃和直肠。4种性类固醇激素受体在牛蛙胃肠胰内的分布表明,性类固醇激素对牛蛙消化功能特别是胃和直肠的功能具有多方面的调节作用。
英文摘要:
      This study investigated the distribution, localization and expression of 4 kinds of sex-steroid hormone receptors in 7 parts of the gastro-entero-pancreatic system in bullfrog, Rana catesbeiana, and discusseds the roles of these 4 kinds of sex-steroid hormone receptors. Tissue samples were taken from 7 parts of the gastro-entero-pancreatic system including esophagus, stomach, duodenum, jejunum, ileum, rectum and pancreas. Paraffin sections and Strept Avidin Biotin-peroxidase Complex (SABC) immunohistochemistry techniques were used to reveal 4 kinds of sex-steroid hormone receptors with antisera including rabbit anti-estrogen receptor α (ERα), estrogen receptor β (ERβ), androgen receptor (AR), progesterone receptor (PR) primary antibodies. Sections were re-stained by haematoxylin to localize the distribution of sex-steroid hormone receptors. Sections not re-stained by haematoxylin were used to analyze the optical density of positive reaction products, and the mean optical density was calculated by Image-Pro Plus. All the data of mean optical density were analyzed by using SPSS 19.0. Mean optical density values of each sex-steroid hormone receptor in 7 parts were analyzed using one-way ANOVA. The results showed that all 4 kinds of sex-steroid hormone receptors were expressed in the gastro-entero-pancreatic system of bullfrog, and there was no obvious difference in the distribution pattern between female and male bullfrogs. Estrogen receptor α (ERα) mainly distributed in stomach gland, lamina propria of rectum and pancreas (Fig. 1). Estrogen receptor β (ERβ) mainly distributed in esophagus epithelium, lamina propria of rectum and pancreas (Fig. 2). Androgen receptor (AR) mainly distributed in esophagus epithelium, rectum and pancreas (Fig. 3). Progesterone receptor (PR) mainly distributed in esophagus epithelium, jejunum and pancreas (Fig. 4). Negative controls of esophagus, stomach, rectum and pancreas staining were also displayed (Fig. 5). Optical density analysis (Table 1) displayed that estrogen receptor α (ERα) had the strongest positive reaction in the stomach and rectum of bullfrog, and relatively weaker reaction in the pancreas. Estrogen receptor β (ERβ) had the strongest positive reaction in the rectum, and relatively weaker reaction in the esophagus and pancreas. Androgen receptor (AR) had the strongest positive reaction in the esophagus, and relatively weaker reaction in the rectum and pancreas. Progesterone receptor (PR) had the strongest positive reaction in the jejunum and relatively weaker reaction in esophagus and pancreas. The positive immunoreactivity of estrogen receptor α (ERα) in stomach and androgen receptor (AR) in esophagus, rectum was stronger in the female than in the male. The positive reaction of estrogen receptor β (ERβ) in pancreas was stronger in the male than in the female. Finally, we summarized that estrogen receptor α (ERα) and estrogen receptor β (ERβ) distributed most predominantly in the gastro-entero-pancreatic system, followed by androgen receptor (AR) and progesterone receptor (PR). And these 4 kinds of sex steroid receptors were mainly distributed in the esophagus, stomach, rectum and pancreas, especially in stomach, and rectum. The distribution of 4 kinds of sex-steroid hormone receptors in the gastro-entero-pancreatic system of bullfrog implicated that sex-steroid hormones might have multiple regulatory roles in the digestive function of bullfrog, especially in the function of stomach and rectum.
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