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陈炼,邬婷,陈燏,邱怡妮,朱善良,祁雯,卫蔚,刘少华,刘青,赵雨恬,王仁雷.2019.温度对福寿螺抗氧化酶活性和 丙二醛含量的影响.动物学杂志,54(5):727-735.
温度对福寿螺抗氧化酶活性和 丙二醛含量的影响
Effects of Temperature Stress on Antioxidase Activity and Malondialdehyde in Pomacea canaliculata
投稿时间:2018-04-11  修订日期:2019-08-29
DOI:10.13859/j.cjz.201905014
中文关键词:  福寿螺  温度胁迫  抗氧化物酶  丙二醛
英文关键词:Pomacea canaliculata  Temperature stress  Antioxidant enzyme  Malondialdehyde
基金项目:国家自然科学基金项目(No. 31770402),江苏省自然科学基金项目(No. BK20171407),江苏省第五期333工程培养对象项目,国家科技支撑计划项目(No. 2015BAD08B01),生物多样性保护重大工程专项基金项目
作者单位E-mail
陈炼 南京林业大学生物与环境学院 南京 210037江苏省生物功能分子重点建设实验室 南京 210013 chenlian_2004@163.com 
邬婷 江苏第二师范学院生命科学与化学化工学院 南京 210013 18795878259@163.com 
陈燏 江苏省生物功能分子重点建设实验室 南京 210013 江苏第二师范学院生命科学与化学化工学院 南京 210013 cysl.1018@163.com 
邱怡妮 江苏第二师范学院生命科学与化学化工学院 南京 210013 qyn_425@163.com 
朱善良 江苏省生物功能分子重点建设实验室 南京 210013 江苏第二师范学院生命科学与化学化工学院 南京 210013 redkindszhusl@163.com 
祁雯 江苏第二师范学院生命科学与化学化工学院 南京 210013 qiwen11070514@163.com 
卫蔚 江苏省生物功能分子重点建设实验室 南京 210013 江苏第二师范学院生命科学与化学化工学院 南京 210013 iamwei.w@163.com 
刘少华 江苏省生物功能分子重点建设实验室 南京 210013 江苏第二师范学院生命科学与化学化工学院 南京 210013 bclsh@jssnu.edu.cn 
刘青 江苏省生物功能分子重点建设实验室 南京 210013 江苏第二师范学院生命科学与化学化工学院 南京 210013 yunyingcao@126.com 
赵雨恬 江苏第二师范学院生命科学与化学化工学院 南京 210013 zcbzyt@126.com 
王仁雷 江苏省生物功能分子重点建设实验室 南京 210013 江苏第二师范学院生命科学与化学化工学院 南京 210013 wrl3501988@163.com 
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中文摘要:
      福寿螺(Pomacea canaliculata)是外来入侵物种,对中国南方大部分地区的生态环境造成严重威胁,其生长和繁殖受到温度影响。本文研究了15 ℃(低温)、25 ℃(对照组)和36 ℃(高温)三种温度条件下,不同时间点(0、6、12、24、48、72 h)福寿螺体内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量的变化。实验结果表明,在低温(15 ℃)和高温(36 ℃)胁迫下,福寿螺肝胰脏和鳃中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活性以及丙二醛含量均呈现先升高后降低的趋势,并且在72 h时恢复到对照组(25 ℃)水平。低温和高温胁迫下,福寿螺鳃中的过氧化氢酶活性和肝胰脏中的超氧化物歧化酶活性在12 h、24 h和48 h时均显著高于对照组(P < 0.05),并在48 h时达到最大值(P < 0.01)。鳃和肝胰脏中的谷胱甘肽过氧化物酶活性在12 h升高并在24 h时达到最大值。低温和高温胁迫下,丙二醛含量分别在24 h和12 h达到最大值。结果表明,温度能够诱导福寿螺抗氧化应激反应。福寿螺可以通过增加体内抗氧化物酶活力来缓解温度胁迫所造成的压力。鳃中抗氧化物酶活性变化快于肝胰脏抗氧化物酶活性变化,福寿螺氧化应激反应表现出组织特异性。
英文摘要:
      Temperature is an important environmental factor that affects the distribution of species and causes the physiological stress response of species. After being introduced into China, the invasive species, Pomacea canaliculata has become a serious pest of important agricultural crops as well as a threat to ecosystems in its invaded regions. Effects of temperature on antioxidant enzyme activities and the malondialdehyde (MDA) contents in the hepatopancreas and gill of P. canaliculata were investigated. P. canaliculata were acclimated for two weeks with temperature 25 ℃. After acclimation, the low and high temperature stress experiments were conducted. The temperature maintaining at 15 ℃ was designated as low-temperature group, 36 ℃ as high-temperature group,and 25 ℃ as control group, with three replicates for each group.All data were analyzed and compared with one-way ANOVA and Duncan using SPSS 22.0. The results showed that the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and the malondialdehyde contents in the hepatopancreas and gill of P. canaliculata changed similarly, both increased first and then decreased under low or high temperature stress (Fig.1﹣8). In groups of 15 ℃ and 36 ℃, CAT activity in gill and SOD activity in hepatopancreas were significant higher than in the control group after 6 h (P < 0.05) and at the highest level at 48 h (P < 0.01, Fig. 2, 3). The activity of GSH-Px in gill and hepatopancreas increased at 12 h and reached the highest level in both low and high temperature groups at 24 h (Fig. 5, 6). MDA contents in gill and hepatopancreas of P. canaliculata were at the highest in the group of 15 ℃ at 24 h and group of 36 ℃ at 12 h, respectively (Fig. 7, 8). There were no significant differences of SOD, CAT, GSH-Px activities and MDA contents between temperature treatment groups and 25 ℃ control group at 72 h (P > 0.05). Furthermore, gills appeared more sensitive to defense oxidative damages than hepatopancreas. The results suggest that temperature stimulation can activate antioxidant enzymes activities in the hepatopancreas and gill of P. canaliculata, and eliminate reactive oxygen species (ROS) induced by low and high temperature stress.
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