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刘丽,潘杰,邓登,成永旭,吴旭干.2018.中华绒螯蟹肉碱乙酰转移酶(CAT)基因表达分析.动物学杂志,53(6):951-962.
中华绒螯蟹肉碱乙酰转移酶(CAT)基因表达分析
Expression of Carnitine Acetyltransferase (CAT) in Chinese Mitten Crab Eriocheir sinensis
投稿时间:2018-05-04  修订日期:2018-10-22
DOI:10.13859/j.cjz.201806013
中文关键词:  中华绒螯蟹  肉碱乙酰转移酶  表达  卵巢发育
英文关键词:Eriocheir sinensis  Carnitine acetyltransferase (CAT)  Expression  Ovarian development
基金项目:国家自然科学基金项目(No. 31572630),农业部现代农业产业技术体系专项资金项目(No. CARS-48),深圳市澳华农牧有限公司横向项目(No. D-8006-15-0054),江苏省海洋与渔业科技创新和推广项目(No. Y2017-4)
作者单位E-mail
刘丽 上海海洋大学 765492348@qq.com 
潘杰 上海海洋大学 984520934@qq.com 
邓登 深圳市澳华农牧有限公司 dengd@alphafeed.com 
成永旭 上海海洋大学 yxcheng@shou.edu.cn 
吴旭干 上海海洋大学 xgwu@shou.edu.cn 
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中文摘要:
      肉碱乙酰转移酶(CAT)是一种作用于短链脂肪酸的酶。本研究克隆了中华绒螯蟹(Eriocheir sinensis)CAT基因的开放阅读框序列(ORF),并采用实时荧光定量PCR(q-PCR)分析其在雌雄不同组织中的表达模式以及不同卵巢发育时期肝胰腺和卵巢的表达变化。结果表明,CAT的ORF长为1 929 bp,编码642个氨基酸,跨膜域和信号肽,预测的蛋白含有一个Acyltransferases ChoActase保守结构域(40 ~ 56 aa),和一个Carn-acyltransf superfamily典型结构域(43 ~ 626 aa)。CAT在雌雄蟹所有组织中均有表达,雄蟹中肝胰腺和肌肉组织的表达量较高,在其他组织中的表达量相对较低;雌蟹中肝胰腺的表达量最高,其次为卵巢和肌肉。肝胰腺以及卵巢的CAT表达量在不同卵巢发育时期之间差异显著(P < 0.05),表明CAT在卵巢发育过程中起着重要作用。
英文摘要:
      Carnitine acetyltransferase (CAT) is an enzyme that has effect on short-chain fatty acids. In this study, the open reading frame (ORF) of CAT was cloned from Chinese mitten crab Eriocheir sinensis by PCR, to analyze the CAT tissue-specific expression and the changing patterns of CAT expression levels in hepatopancreas and ovaries during different stages of ovarian development by quantitative real-time PCR (q-PCR). The results showed that the ORF of CAT was 1 929 bp in length and encoded 642 amino acids (Fig. 1) without transmembrane domain and signal peptide, with the predicted protein containing an Acyltransferases ChoActase conserved domain (40﹣56 aa) and a typical Carn-acyltransf superfamily domain (43﹣626 aa) (Fig. 2). For either males or females, CAT expressed in all detected tissues. In males, higher CAT expression levels were detected in hepatopancreas and muscle (Fig. 4). In females, the highest expression level was obseeved in hepatopancreas, followed by the ovaries and muscle (Fig. 5). The observed changes of CAT expression levels in hepatopancreas and ovaries significantly varied in different ovarian development stages (P < 0.05) (Fig. 6, 7), suggesting that CAT may play an important role in E. sinensis during its ovarian development.
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