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吴晓雯,余海,陈青萍,彭欣,张华伟,张永普.2019.基于COI基因对温州沿海岛屿疣荔枝螺遗传多样性研究.动物学杂志,54(02):254-269.
基于COI基因对温州沿海岛屿疣荔枝螺遗传多样性研究
Genetic Diversity of Reishia clavigera on the Coastal Islands of Wenzhou Based on COI Gene
投稿时间:2018-08-10  修订日期:2019-02-19
DOI:10.1385.9/j.cjz.201902012
中文关键词:  疣荔枝螺  线粒体DNA  单倍型  地理谱系结构  温州沿海
英文关键词:Reishia clavigera  Mitochondrial DNA  Phylogeographical structure  Haplotypes  Wenzhou coastal
基金项目:浙江省科技计划项目(No. 2016F50031),温州大学研究生创新基金项目(No. 46);
作者单位E-mail
吴晓雯 温州大学 xwen0106@126.com 
余海 苍南县水产研究所 cnyuhai@126.com 
陈青萍 温州大学 785950604@qq.com 
彭欣 浙江省海洋水产养殖研究所 pengxin_1128@163.com 
张华伟 浙江省海洋水产养殖研究所 zzz-hw@163.com 
张永普 温州大学 zhangypu@126.com 
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中文摘要:
      温州沿海岛屿众多,水文环境复杂,疣荔枝螺(Reishia clavigera)是岩相潮间带常见种,对其群体间遗传多样性的分析,有助于合理保护和开发利用种质资源。本实验对温州沿海14个典型岛屿的158个疣荔枝螺个体,进行细胞色素氧化酶亚基Ⅰ(COⅠ)基因片段序列测定,得到158 条长度为672 bp的序列,A、T、C、G 四种碱基含量分别为23.0%、38.4%、17.4%和21.2%。共检测到保守位点562个,变异位点108 个,其中包括简约信息位点57 个,单突变位点51 个。所有个体的核苷酸多样性指数Pi为0.008 0 ± 0.029 7,单倍型多样性为指数Hd为0.978 ± 0.006,平均核苷酸差异度为5.328。群体间遗传距离在0.005 7 ~ 0.011 1之间,群体内遗传距离在0.005 7 ~ 0.010 8之间,不同疣荔枝螺群体之间的遗传距离处于同一水平。霓屿岛和大竹峙岛群体内遗传距离最小,洞头岛群体内遗传距离最大。共检测到102 个单倍型,大部分单倍型聚合为一支,没有形成明显的区系结构,各个岛屿的疣荔枝螺存在基因交流。
英文摘要:
      Reishia clavigera (Mollusca: Muricidae) is a common species in the rocky intertidal zone along the coastal islands of Wenzhou, Zhejiang Province in China. There are many islands and their hydrological environments are complex in coastal region of Wenzhou. In order to investigate the genetic diversity and population structure of R. clavigera, we collected 158 individuals from 14 populations (Table 1 and Fig. 1). We amplified 672 bp length of mitochondrial cytochrome c oxidase submitⅠ(COⅠ) gene (primer LCOⅠ 1490 5′-GGT CAA CAA ATC ATA AAG ATA TTG G-3′and HCOI2198 5′-TAA ACT TCA GGG TGA CCA AAA AAT CA-3′), which could provide the basic data on their resources and genetic utilization. The ratios of A, T, C, G were 23.0%, 38.4%, 17.4% and 21.2% (Table 2),respectively, which was in accordance with the mitochondrial composition. All sequences obtained included 108 polymorphism sites (57 parsim-information sites and 51 singleton sites) (Table 2). The nucleotide diversity was 0.008 0, while haplotypes diversity and the average number of nucleotide differences were 0.978 and 5.328, respectively (Table 2). A total of 102 haplotypes were detected (Table 3). The genetic distance between populations ranged from 0.005 7 to 0.011 1, and the genetic distance within the population ranged from 0.005 7 to 0.010 8 (Table 4). The genetic distance between groups from Niyu Island and Dazhushi Island was minimal, and the genetic distance within groups from Dongtou Island was maximal. The correlation between genetic distance and geographical distance based on COⅠ gene fragments was significant (R2 = 0.134, P < 0.01), but there was no geographical isolation (r = 0.365, P = 0.11) (Fig. 2). The topological structure of R. clavigera in ML analysis had no obvious geographical structure (Fig. 3 and Fig. 4).
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