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王新栋,孙雪婧,刘恩雪,赵巧雅,董萍萍,陈秋生,林金杏.2019.斑马鱼脾显微与超微结构的研究.动物学杂志,54(02):222-235.
斑马鱼脾显微与超微结构的研究
Microstructure and Ultrastructure of Zebrafish Spleen
投稿时间:2018-09-12  修订日期:2019-02-14
DOI:10.1385.9/j.cjz.201902009
中文关键词:  斑马鱼    显微结构  超微结构
英文关键词:Zebrafish, Danio rerio  Spleen  Microstructure  Ultrastructure
基金项目:上海市科委科研计划项目(No. 16140904300,15140901000)
作者单位E-mail
王新栋 南京农业大学动物医学院 514795402@qq.com 
孙雪婧 南京农业大学动物医学院 544879429@qq.com 
刘恩雪 南京农业大学动物医学院 1228140646@qq.com 
赵巧雅 南京农业大学动物医学院 1983400529@qq.com 
董萍萍 南京农业大学动物医学院 1799355371@qq.com 
陈秋生 南京农业大学动物医学院 chenqsh305@njau.edu.cn 
林金杏 上海实验动物研究中心 linjinxing83@163.com 
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中文摘要:
      应用光学显微镜和透射电镜对斑马鱼(Danio rerio)脾的显微与超微结构进行了观察。光镜结果表明,斑马鱼的脾主要由脾髓和网状组织两部分构成,脾髓分布于整个脾,分为白髓和红髓,但界限不明显,呈混合分布。红髓染色相对较浅,占脾实质的大部分区域,主要由密集的红细胞构成,可见脾窦结构,脾索结构不明显。白髓染色较深,主要由密集的淋巴细胞构成,淋巴细胞的胞核染色较深,呈深紫色。经Gordon-Sweet银染显示网状纤维后,可清晰观察到脾小结,从而可清晰区分红髓与白髓,脾小结与哺乳动物的类似,主要是由密集的淋巴细胞构成。酸性磷酸酶染色可见斑马鱼脾内有大量吞噬性细胞存在。电镜结果显示,红髓分布有不同类型的红细胞、血小板和浆细胞,白髓分布有淋巴细胞、巨噬细胞、单核细胞、嗜中性粒细胞、浆细胞和网状细胞,淋巴细胞胞质内含有多泡体,这可能与淋巴细胞发挥免疫功能有着密切联系。斑马鱼脾与大多数硬骨鱼相似,未观察到椭球这一特殊结构。
英文摘要:
      The microstructure and ultrastructure of zebrafish (Danio rerio) spleen were observed by light microscopy and transmission electron microscopy. The results showed that the spleen of zebrafish mainly consisted of splenic pulp and reticular tissue. The splenic pulp was distributed in the whole spleen, which could be divided into white pulp and red pulp. The red and white pulps showed mixture distribution without obvious boundary (Fig. 3). The red pulp accounted for most of the spleen parenchyma, which was mainly composed of dense red blood cells. The splenic sinus was observed, while the splenic cord was not obvious. The white pulp with deeper staining was mainly composed of dense lymphocytes. The nuclei of lymphocytes were stained dark purple. After Gordon-Sweet silver staining to show reticular fibers, splenic nodules could be clearly observed (Fig. 4). Therefore, the red pulp and the white pulp could be clearly distinguished. Splenic nodules were similar with those of mammals, which were mainly composed of dense lymphocytes. Acid phosphatase staining revealed that there were many phagocytic cells in zebrafish spleen (Fig. 4). Electron microscopy showed the red and white pulp ultrastructure of the spleen. There were different types of red blood cells, platelets and plasma cells in the red pulp, and there were lymphocytes, macrophages, monocytes, neutrophils, plasmocytes and reticular cells in the white pulp. The structure of ellipsoid was not observed in the spleen. Multiple vesicles were observed in the lymphocyte cytoplasm, which may be closely related to the immune function of lymphocytes (Fig. 5﹣10). The spleen of zebrafish was similar to that of most bony fish, and the ellipsoid was not observed.
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