| 路生鑫,李丕鹏,陆宇燕,于奇令,周正彦.2019.人工养殖条件下三种锦蛇的粪便微生物多样性.动物学杂志,54(2):270-278. |
| 人工养殖条件下三种锦蛇的粪便微生物多样性 |
| Fecal Microbial Diversity of Three Species of Elaphe in Captive Breeding |
| 投稿时间：2018-09-25 修订日期：2019-01-30 |
| DOI：10.1385.9/j.cjz.201902013 |
| 中文关键词: 锦蛇 粪便 微生物多样性 |
| 英文关键词:Elaphe Faeces Microbial diversity |
| 基金项目:国家林业局项目（No. 2130211） |
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| 中文摘要: |
| 采用高通量测序技术，分析比较了实验室人工饲养的王锦蛇（Elaphe carinata）、棕黑锦蛇（E. schrenckii）和赤峰锦蛇（E. anomala）3种锦蛇粪便样本的微生物多样性。结果显示，在送检的粪便样本中，Shannon多样性指数（P < 0.05）和Simpson多样性指数（P < 0.05）在王锦蛇和棕黑锦蛇之间有显著性差异，赤峰锦蛇与前两者均没有差异；ace与chao1指数在3种锦蛇之间没有显著性差异。共检测出8 个菌门，厚壁菌门（Firmicutes）、拟杆菌门（Bacteroidetes）和变形菌门（Proteobacteria）是3种锦蛇共享菌门。在属水平上3 种锦蛇的优势菌群明显不同，志贺氏菌属（Shigella）、梭状芽胞杆菌属（Clostridium_sensu_stricto_11）及拟杆菌属（Bacteroides）分别是王锦蛇、棕黑锦蛇和赤峰锦蛇粪便样本中相对丰度最高的菌属。3 种锦蛇粪便微生物多样性存在着明显的差异，但其是否可以作为区分这3种锦蛇的依据还需要进一步研究。 |
| 英文摘要: |
| We investigated fecal microflora in the fecal samples of three species of Elaphe (E. carinata, E. schrenckii and E. anomala) which were fed in captivity in laboratory by using a V4﹣V5 region of 16S rRNA gene amplicon sequencing and analyzed the diversity and composition of the microflora. The results showed that the number of OUTs was 192 and the number of shared OTUs was 116 in all samples (Fig. 1). The diversity indexes (Shannon and Simpson) were significantly different between the E. carinata and the E. schrenckii, but the E. anomala had no significant difference when compared to the above two species. There was no significant difference in ace and chao1 among the three species (Table 1). Eight phyla were detected in the samples, but only three phyla, Firmicutes, Bacteroidetes and Proteobacteria were shared by these three
species (Fig. 3). The ranking of relative abundance of the three phyla was obviously different. There were 4 genera (Bacteroides, norank_f_Porphyromonadaceae, Enterococcus, Escherichia-Shigella), and the average relative abundance was 77.92% in the E. carinata faeces, there were 9 genera (Bacteroides, Bacillus, Enterococcus, Clostridium_sensu_stricto_1, Clostridium_sensu_stricto_11, Paeniclostridium, Aeromonas, Escherichia-Shigella, Proteus, total of average relative abundance is 75.53%) in the E. schrenckii faeces. There were 6 genera (Bacteroides, norank_f_Porphyromonadaceae, Enterococcus, Clostridium_sensu_stricto_1, Edwardsiella, Escherichia-Shigella), and the average relative abundance was 67.42% in the E. anomala faeces (Table 2). Notably, Escherichia-Shigella had the highest relative abundance in E. carinata faeces;
Clostridium_sensu_stricto_11 had the highest relative abundance in E. schrenckii faeces; Bacteroides had the highest relative abundance in E. anomala faeces. There is significant difference in the composition of microbial communities in the faeces among the three species. Under the same feeding environment and food condition, the main factors determining the difference of the microbial communities may be closely related to the specific genomes of each species, which needs further clarification. |
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