Abstract:The Chinese endemic piebald odorous frogs Odorrana schmackeri species complexare widely distributed in Southern China. However, their taxonomy is still unresolved to date. Previous analyses indicated the existence of many cryptic species in this complex. Due to the absence of obvious geographical barrier and morphological similarities, there are still some controversies on the species composition, distribution boundaries, geographical distribution patterns of the Odorrana schmackeri species complex in Fujian and Zhejiang hill regions. Here, field surveys were conducted in Pingnan County of Ningde City and Pucheng County of Nanping City, Fujian Province from September to October in 2016 (Fig. 1). Species delimitation analyses were first conducted based on the data of both morphological characteristics and comparisons (Table 2). In addition, the mitochondrial 12S and 16S rRNA gene fragments were amplified, sequenced and aligned with both sequences from O. tianmuii, O. huanggangensis and O. schmackeri. Molecular phylogenetic analyses and the genetic distance estimation were then conducted based on these data. The topology of phylogenetic tree indicated that samples from Pingnan county were clustered with O. huanggangensis from Wuyi mountain of Fujian province with strong supports, with genetic distance less than 0.1% (Fig. 3, Table 3). In addition, phylogenetic analyses strongly supported the clustering of samples from Pucheng county with O. tianmuii from Tianmu mountain in Zhejiang province withgenetic distance of 0.4% (Fig. 3, Table 3). Therefore, the Odorrana schmackeri species complex distributed in Fujian province consist of two cryptic species, i.e., O. huanggangensis and O. tianmuii. O. huanggangensis is mainly distributed in Wuyi mountain, Minjiang River and Chiu-lung River basin, whereas O. tianmuii is only distributed in northeast Guancuo Town in Pucheng County, where is the second headstream of Qujiang River and one of the main tributaries of Qiantang River. The distribution of O. tianmuii in Fujian province revealed in this study is a new record, and the results here further enriched the species diversity of amphibian in Fujian Province. Further studies should be focused on the species composition of the O. schmackeri species complex in the Yangtze River basin of northwest Pucheng County and whether the river systems and drainage basins influenced the species differentiation and speciation.