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张晨,刘斯汝,蔡勇,李囿蓉,何灵芝,蔡建亮,罗文学,阿依木古丽.2019.藏山羊与藏绵羊肺组织结构的对比研究.动物学杂志,54(5):687-692.
藏山羊与藏绵羊肺组织结构的对比研究
Comparison of Lung Microstructure between Tibetan Goat and Sheep
投稿时间:2019-03-27  修订日期:2019-08-25
DOI:10.13859/j.cjz.201905009
中文关键词:  藏山羊  藏绵羊    弹力纤维
英文关键词:Tibetan goat  Tibetan sheep  Lung  Elastic fibers
基金项目:中央高校基本业务费专项资金(31920190024,31920190020),甘肃省高等学校科研项目(2018B-027)
作者单位E-mail
张晨 西北民族大学生命科学与工程学院 兰州 730030 841371440@qq.com 
刘斯汝 西北民族大学生命科学与工程学院 兰州 730030 841371440@qq.com 
蔡勇 西北民族大学实验教学部 兰州 730030 841371440@qq.com 
李囿蓉 西北民族大学生命科学与工程学院 兰州 730030 841371440@qq.com 
何灵芝 西北民族大学生命科学与工程学院 兰州 730030 841371440@qq.com 
蔡建亮 西北民族大学生命科学与工程学院 兰州 730030 18974576@qq.com 
罗文学 天祝藏族自治县畜牧技术推广站 武威 73320 841371440@qq.com 
阿依木古丽 西北民族大学生命科学与工程学院 兰州 730030 841371440@qq.com 
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中文摘要:
      为了探讨藏山羊(Capra hircus)与藏绵羊(Ovis aries)在高原低氧环境中肺组织结构的差异,采用Gomori醛品红染色及H.E染色对藏山羊和藏绵羊肺组织进行对比研究。结果表明,藏山羊与藏绵羊肺被膜厚度无显著差异(P > 0.05),但肺被膜中弹力纤维藏山羊显著多于藏绵羊(P < 0.05)。肺泡面积藏山羊与藏绵羊无显著差异(P > 0.05),但肺泡隔宽度和肺泡隔中毛细血管的数量藏山羊显著高于藏绵羊(P < 0.05)。肺细支气管黏膜皱襞厚度藏山羊与藏绵羊无显著差异(P > 0.05),但细支气管平滑肌厚度藏山羊显著高于藏绵羊,细支气管黏膜上皮1 mm2中杯状细胞数量藏山羊显著高于藏绵羊(P < 0.05)。外径小于100 μm的肺微动脉中,藏山羊血管平滑肌占外径百分比显著高于藏绵羊(P < 0.05),而当外径大于100 μm时,两者间差异不显著(P > 0.05)。
英文摘要:
      The Gomori and H.E staining were used to explore the difference in lung microstructure between Tibetan goat (Capra hircus) and Tibetan sheep (Ovis aries). The results showed that there was no significant difference in lung capsule thickness between these two species (P > 0.05), but the number of elastic fibers in Tibetan goat lung capsule was more than that in Tibetan sheep (P < 0.05) (Fig.1 and Table 1). There was no significant difference in alveolar area (P > 0.05), but the alveolar septum of Tibetan goat was significantly wider with much more capillaries than that of Tibetan sheep (P < 0.05) (Table 2). There was no significant difference in the thickness of bronchioles plica, but the bronchiole smooth muscle of Tibetan goat was significantly thicker than that of Tibetan sheep (P < 0.05)(Fig.2 and Table 3). The average number of goblet cells in the bronchioles mucosa epithelium of Tibetan goat was significantly more than that in Tibetan sheep (P < 0.05) (Table 3). In addition, the percentage of vascular smooth muscle in the pulmonary arteriole with diameter less than 100 μm was significantly higher in Tibetan goat than that in Tibetan sheep (P < 0.05), while the difference was not significant when the pulmonary arteriole diameter was greater than 100 μm (P > 0.05) ( Table 4).
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