| 张盈盈,田兰香.2019.亚磁场对雄性小鼠神经递质、 激素和血常规的影响.动物学杂志,54(4):538-548. |
| 亚磁场对雄性小鼠神经递质、 激素和血常规的影响 |
| Effects of Hypomagnetic Field on Neurotransmitter, Hormone and Blood Parameters in Male Mice |
| 投稿时间：2019-04-08 修订日期：2019-06-04 |
| DOI：10.13859/j.cjz.201904011 |
| 中文关键词: 亚磁场 地磁场 神经递质 激素 血常规 |
| 英文关键词:Hypomagnetic field Geomagnetic field Neurotransmitters Hormones Blood parameters |
| 基金项目:中国科学院重点资助项目,国家自然科学基金项目（面上项目，重点项目，重大项目） |
|
| 摘要点击次数: 1063 |
| 全文下载次数: 1062 |
| 中文摘要: |
| 本研究主要是探讨连续亚磁场环境暴露对C57BL/6雄性小鼠（Mus musculus domesticus）神经递质、激素含量和血常规参数的影响。实验组为亚磁场暴露组（1.1 ± 0.5）μT，对照组为地磁场组（53.3 ± 0.2）μT。实验对象分别为3周龄幼年小鼠和8周龄成年小鼠。幼年小鼠随机分为实验组与对照组，每组42 只；成年小鼠也随机分为实验组与对照组，每组36只。幼年小鼠在两种磁场环境中的暴露时间为1 ~ 5周，成年小鼠的暴露时间为1、3、6、9、12周。采用液相色谱-质谱联用法（LC-MS）检测小鼠脑组织及血清中的神经递质含量，包括5-羟色胺（5-HT）、多巴胺（DA）、乙酰胆碱（Ach）、去甲肾上腺素（NA）、γ-氨基丁酸（GABA）；用 ELISA 方法检测小鼠血清中的激素含量，包括甲状腺素（T4）、生长激素（GH）、去甲肾上腺素（NA）；血常规在北京大学医学部检测。采用独立样本 t 检验对实验组与对照组的检测结果进行差异分析。与地磁场组相比，亚磁场组幼年小鼠脑组织多巴胺（DA）含量在第5周有显著降低（P < 0.05），血清中的甲状腺素（T4）含量在第 2和5 周有显著降低（P < 0.05），去甲肾上腺素（NA）含量在第4周出现显著性降低（P < 0.01）；与地磁场组相比，成年小鼠脑组织5-羟色胺（5-HT）、去甲肾上腺素（NA）含量在处理1周时均出现显著性降低（P < 0.05），血清中5-羟色胺（5-HT）含量在第3周也出现显著性降低（P < 0.01），甲状腺素（T4）含量在第 3、6、12 周出现显著性降低（P < 0.05）。血常规结果显示，成年和幼年小鼠的白细胞（WBC）在暴露早期第1周和第2周分别出现显著性增多（P < 0.01），其他参数基本无变化。本研究发现，两个年龄段的小鼠对亚磁场响应的指标不完全一致，仅甲状腺素（T4）在两个年龄段小鼠的体内都出现显著性降低。通过本研究说明，甲状腺素（T4）、5-羟色胺（5-HT）、去甲肾上腺素（NA）、多巴胺（DA）和白细胞（WBC）可能是不同年龄段小鼠对亚磁场环境暴露较为敏感的生理指标，可在今后的亚磁场生物效应研究中重点关注。 |
| 英文摘要: |
| We aimed to investigate the effects of continuous exposure to hypomagnetic field on neurotransmitter, hormone content and blood routine parameters in juvenile (three weeks old) and adult (eight weeks old) male C57BL/6 mice (Mus musculus domesticus). The experimental group was exposed to the hypomagnetic field at density of 1.1 ± 0.5 μT, while the control group was exposed to the geomagnetic field at density of 53.3 ± 0.2 μT. A total of 84 and 72 male juvenile and adult mice were randomly divided into the experimental group and the control group, 42 and 36 in each group, respectively. Juvenile mice were exposed to the two kinds of magnetic field for 1 to 5 weeks while adult mice for 1, 3, 6, 9, and 12 weeks. The contents of neurotransmitters and hormones including gamma-aminobutyric acid (GABA), serotonin (5-HT), dopamine (DA), acetylcholine (Ach), noradrenaline (NA), thyroxine (T4) and growth hormone (GH) in the brain tissue or serum samples were measured using LC-MS and ELISA. Blood routine parameters were also measured in Peking University Health Science Center. We compared the results of the experimental and control groups with independent sample t-test. The results showed that the contents of 5-HT and NA in the brain tissues of adult mice exposed to hypomagnetic field decreased significantly after 1-week exposure (P < 0.05) (Fig. 1), and the contents of 5-HT in serum decreased significantly after 3-week exposure (P < 0.01). The contents of T4 decreased significantly at the 3rd, 6th, 12th week (P < 0.05) (Fig. 2). The content of DA in brain tissues of juvenile mice decreased at the 5th week (P < 0.05) (Fig. 1). The content of T4 in serum decreased significantly at the 2nd and 5th week (P < 0.05) while NA decreased significantly at the 4th week (P < 0.01) (Fig. 2). Blood routine examination showed that white blood cells (WBC) increased significantly at the 1st week for adult and 2nd week for juvenile mice, respectively (P < 0.01). Other parameters had no significant differences (Fig. 3 and appendix 1). This study shows that the parameters responding to the exposure to hypomagnetic field for juvenile and adult mice are not completely consistent, excepting that T4 reduces significantly in both groups. The results indicate that T4, 5-HT, NA, DA and WBC may be physiological parameters sensitive to the hypomagnetic-field exposure, which should be paid special attention to in future studies. |
| 附件 |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
